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PCG3003

Sigma-Aldrich

TruPAGE Tris-MOPS SDS Express Running Buffer

20 ×

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NACRES:
NB.22

concentration

20 ×

compatibility

for use with Sigma-Aldrich TruPAGE Precast Gels

storage temp.

room temp

Related Categories

Application

TruPAGE Tris-MOPS SDS Express Running Buffer has been used to separate proteins through sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE).

Features and Benefits

TruPAGE Tris-MOPS SDS Express Running Buffer is specially formulated to be used with TruPAGE Precast Gels. This running buffer will provide increased resolution for small MW proteins, and will noticeably shorten the run time. This running buffer needs to be supplemented with a 1X working concentration of TruPAGE Antioxidant (PCG3007) to prevent protein reoxidation during electrophoresis when running reduced protein samples. Please refer to the gel migration chart to select the appropriate precast gel and running buffer combination for your experimental needs.

Legal Information

TruPAGE is a trademark of Sigma-Aldrich Co. LLC

Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 1

Flash Point(C)

Not applicable


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Detection of phosphorylated Akt and MAPK in cell culture assays.
Molgaard S, et al.
MethodsX, 3, 386-398 (2016)
Simon Molgaard et al.
MethodsX, 3, 386-398 (2016-06-09)
This article describes an immunocytochemistry (ICC) method for staining against phosphorylated forms of the kinases Akt (pAkt) and MAPK (pMAPK). Phosphorylation is induced upon their activation by a number stimuli including insulin and brain-derived neurotrophic factor (BDNF), and is prerequisite
John R Christin et al.
Cell reports, 31(10), 107742-107742 (2020-06-11)
Lineage plasticity is important for the development of basal-like breast cancer (BLBC), an aggressive cancer subtype. While BLBC is likely to originate from luminal progenitor cells, it acquires substantial basal cell features and contains a heterogenous collection of cells exhibiting

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