The detergents and chaotropic agents in the kit will at least partially denature the proteins, making them unsuitable for use in co-immunoprecipitation assays.
PE0230
Plant Total Protein Extraction Kit
Suitable for any plant species or tissue
Synonym(s):
Total Protein Extraction Kit
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This Item | PE0220 | PE0240 | XNAP2 |
|---|---|---|---|
| technique(s) protein extraction: suitable | technique(s) - | technique(s) - | technique(s) PCR: suitable |
| storage temp. −20°C | storage temp. −20°C | storage temp. −20°C | storage temp. −20°C |
| Quality Level 200 | Quality Level 200 | Quality Level 200 | Quality Level 200 |
General description
The Plant Total Protein Extraction Kit is designed specifically for use in plant bioscience to extract a qualitative sample of all proteins from any type of plant species or tissue. The protocol does not require any ultracentrifugation or aqueous polymer two-phase partitioning (APTP). The kit includes two reagents; a plant specific protease inhibitor cocktail and a new chaotropic reagent with increased solubilizing power to extract more hydrophobic proteins (C0356, Protein Extraction Reagent Type 4). Routine use of the reagent in sequential protein extraction of a plant tissue sample with fresh reagent removes nearly all protein from the tissue. The protease inhibitor cocktail is a mixture of protease inhibitors with broad specificity for the inhibition of serine, cysteine, metalloproteases, aspartic, and aminopeptidases. It contains pepstatin A, 4-(2-aminoethyl)benzenesulfonyl fluoride (AEBSF), E-64, leupeptin, 1,10-phenanthroline, and bestatin. The cocktail has been demonstrated to be highly effective in preventing protein degradation during the extraction process.
Following removal of polyphenolics, tannins, and other interfering substances, ground plant tissue, fresh or frozen, is resuspended in the chaotropic reagent. Plant debris is pelleted by centrifugation and the protein extract is collected. The end result is a qualitative total protein sample, ready for downstream proteomic analysis.
Following removal of polyphenolics, tannins, and other interfering substances, ground plant tissue, fresh or frozen, is resuspended in the chaotropic reagent. Plant debris is pelleted by centrifugation and the protein extract is collected. The end result is a qualitative total protein sample, ready for downstream proteomic analysis.
Application
Preparation Note
Sufficient for preparation of 20 separate extractions from plant tissue samples of 10-250mg
signalword
Warning
hcodes
Hazard Classifications
Acute Tox. 4 Oral - Aquatic Chronic 2 - Carc. 2 - Repr. 2
Storage Class
11 - Combustible Solids
flash_point_f
185.0 °F - closed cup
flash_point_c
85 °C - closed cup
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Revealing proteins associated with symbiotic germination of Gastrodia elata by proteomic analysis
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Gastrodia elata, a mycoheterotrophic orchid, is a well-known medicinal herb. In nature, the seed germination of G. elata requires proper fungal association, because of the absence of endosperm. To germinate successfully, G. elata obtains nutrition from mycorrhizal fungi such as
Samuel L Chen et al.
Frontiers in plant science, 10, 1116-1116 (2019-10-15)
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E Mazzio et al.
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Production of reactive oxygen species (ROS) can be accelerated under various biotic and abiotic stresses causing lipid peroxidation, protein degradation, enzyme inactivation, and DNA damage. Superoxide reductase (SOR) is a novel antioxidant enzyme from Pyrococcus furiosus and is employed by
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Instructions
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Hi there, I just wanted to find out if the samples extracted from this kit can be used in Co-IP or not?
1 answer-
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Will my protein samples be denatured when using Product PE0230, Plant Protein Extraction Kit?
1 answer-
Yes. The chaotropic agents and the detergent in the kit will denature the proteins at least partially.
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What is the Department of Transportation shipping information for this product?
1 answer-
Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.
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How can I improve my yield when using Product PE0230, Plant Protein Extraction Kit?
1 answer-
A second extraction can be performed and combined with the first to increase protein extracted to about 95% of the total.
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What is the expected protein yield when using Product PE0230, Plant Protein Extraction Kit?
1 answer-
Depending on the tissue used (age, type (leaf, stem, root) health, etc.) one can expect 1-4 mg/mL at about 75-80% of the total protein.
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