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PE0230

Sigma-Aldrich

Plant Total Protein Extraction Kit

Suitable for any plant species or tissue

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NACRES:
NA.56

Quality Level

200

storage temp.

−20°C

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General description

The Plant Total Protein Extraction Kit is designed specifically for use in plant bioscience to extract a qualitative sample of all proteins from any type of plant species or tissue. The protocol does not require any ultracentrifugation or aqueous polymer two-phase partitioning (APTP). The kit includes two reagents; a plant specific protease inhibitor cocktail and a new chaotropic reagent with increased solubilizing power to extract more hydrophobic proteins (C0356, Protein Extraction Reagent Type 4). Routine use of the reagent in sequential protein extraction of a plant tissue sample with fresh reagent removes nearly all protein from the tissue. The protease inhibitor cocktail is a mixture of protease inhibitors with broad specificity for the inhibition of serine, cysteine, metalloproteases, aspartic, and aminopeptidases. It contains pepstatin A, 4-(2-aminoethyl)benzenesulfonyl fluoride (AEBSF), E-64, leupeptin, 1,10-phenanthroline, and bestatin. The cocktail has been demonstrated to be highly effective in preventing protein degradation during the extraction process.

Following removal of polyphenolics, tannins, and other interfering substances, ground plant tissue, fresh or frozen, is resuspended in the chaotropic reagent. Plant debris is pelleted by centrifugation and the protein extract is collected. The end result is a qualitative total protein sample, ready for downstream proteomic analysis.

Application

The plant total Protein Extraction Kit has been used to isolate crude seed protein. It has also been used to extract the total protein.

Quantity

Sufficient for preparation of 20 separate extractions from plant tissue samples of 10-250mg

Kit Components Also Available Separately

Product No.
Description
SDS
  • C0356Protein Extraction Reagent Type 4 1 bottleSDS
  • P9599Protease Inhibitor Cocktail, for plant cell and tissue extracts, DMSO solution 1 mLSDS

Signal Word

Warning

Hazard Classifications

Acute Tox. 4 Oral - Aquatic Chronic 2 - Carc. 2 - Repr. 2

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point(F)

185.0 °F - closed cup

Flash Point(C)

85 °C - closed cup

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25G
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705578-5MG-PW

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MMYOMAG-74K-13

1000309185

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Xing-Min Geng et al.
Frontiers in plant science, 7, 26-26 (2016-02-10)
Production of reactive oxygen species (ROS) can be accelerated under various biotic and abiotic stresses causing lipid peroxidation, protein degradation, enzyme inactivation, and DNA damage. Superoxide reductase (SOR) is a novel antioxidant enzyme from Pyrococcus furiosus and is employed by
Bioactivity-Guided Isolation of Neuritogenic Factor from the Seeds of the Gac Plant (Momordica cochinchinensis)
Mazzio E, et al.
Evidence-Based Complementary and Alternative Medicine : ECAM, 2018 (2018)
E Mazzio et al.
Evidence-based complementary and alternative medicine : eCAM, 2018, 8953958-8953958 (2018-06-30)
Nerve growth factor (NGF) is an endogenously produced protein with the capacity to induce central nervous system (CNS) neuronal differentiation and repair. NGF signaling involves its binding to tropomyosin-related kinase (Trk) receptors, internalization, and initiation of phosphorylation cascades which cause
Xu Zeng et al.
Botanical studies, 59(1), 8-8 (2018-03-08)
Gastrodia elata, a mycoheterotrophic orchid, is a well-known medicinal herb. In nature, the seed germination of G. elata requires proper fungal association, because of the absence of endosperm. To germinate successfully, G. elata obtains nutrition from mycorrhizal fungi such as
Samuel L Chen et al.
Frontiers in plant science, 10, 1116-1116 (2019-10-15)
Pre-mRNA alternative splicing is a conserved mechanism for eukaryotic cells to leverage existing genetic resources to create a diverse pool of protein products. It is regulated in coordination with other events in RNA metabolism such as transcription, polyadenylation, RNA transport
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