PHG0004
BIS-TRIS
Synonym(s):
2,2-Bis(hydroxymethyl)-2,2′,2″-nitrilotriethanol, 2-Bis(2-hydroxyethyl)amino-2-(hydroxymethyl)-1,3-propanediol, Bis(2-hydroxyethyl)amino-tris(hydroxymethyl)methane
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About This Item
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biological source
synthetic
Quality Level
form
powder
technique(s)
cell culture | mammalian: suitable
impurities
Endotoxin, microbial, and trace metals; tested
useful pH range
5.8-7.2
pKa (25 °C)
6.5
solubility
H2O: 0.1 M, clear, colorless
suitability
suitable for manufacturing use
foreign activity
Cytotoxicity, DNase, NICKase, RNase, and Protease; tested
SMILES string
OCCN(CCO)C(CO)(CO)CO
InChI
1S/C8H19NO5/c10-3-1-9(2-4-11)8(5-12,6-13)7-14/h10-14H,1-7H2
InChI key
OWMVSZAMULFTJU-UHFFFAOYSA-N
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This Item | B9754 | B4429 | 14879 |
---|---|---|---|
suitability suitable for manufacturing use | suitability suitable for Western blot, suitable for column chromatography of proteins | suitability - | suitability - |
form powder | form crystalline powder | form crystalline powder | form powder |
biological source synthetic | biological source - | biological source - | biological source - |
technique(s) cell culture | mammalian: suitable | technique(s) UV/Vis spectroscopy: suitable, electrophoresis: suitable | technique(s) cell culture | insect: suitable, cell culture | mammalian: suitable | technique(s) - |
impurities Endotoxin, microbial, and trace metals; tested | impurities - | impurities endotoxin and total aerobic microbial count, tested | impurities insoluble matter, passes filter test |
useful pH range 5.8-7.2 | useful pH range 5.8-7.2 | useful pH range 5.8-7.2 | useful pH range 5.8-7.2 |
General description
M-Clarity Program
Buffer quality is vital for the success of biopharmaceutical processes, because buffers are indispensable in nearly every production step.
Our broad portfolio of buffer materials manufactured under appropriate controls is tailored to your needs. Ranging from non-GMP grades for low-risk application, to IPEC-PQG GMP for higher-risk applications, we have products covering all your manufacturing needs.
Application
Bis Tris is used in a wide variety of biological applications including protein separation buffers, cell culture media, electrophoresis separations and diagnostic reagent formulations.
Legal Information
signalword
Warning
hcodes
Hazard Classifications
Eye Irrit. 2
Storage Class
11 - Combustible Solids
wgk_germany
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
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