PHG0004

BIS-TRIS

• Synonym(s): 2,2-Bis(hydroxymethyl)-2,2′,2″-nitrilotriethanol, 2-Bis(2-hydroxyethyl)amino-2-(hydroxymethyl)-1,3-propanediol, Bis(2-hydroxyethyl)amino-tris(hydroxymethyl)methane
• Empirical Formula (Hill Notation): C₈H₁₉NO₅
• CAS Number: 6976-37-0
• Molecular Weight: 209.24
• Beilstein: 2205275
• EC Number: 230-237-7
• MDL number: MFCD00002853
• PubChem Substance ID: 329823022
• NACRES: NA.21

Properties

• biological source: synthetic
• Quality Level: 400
• form: powder
• technique(s): cell culture | mammalian: suitable
• impurities: Endotoxin, microbial, and trace metals; tested
• useful pH range: 5.8-7.2
• pKa (25 °C): 6.5
• solubility: H₂O: 0.1 M, clear, colorless
• suitability: suitable for manufacturing use
• foreign activity: Cytotoxicity, DNase, NICKase, RNase, and Protease; tested
• SMILES string: OCCN(CCO)C(CO)(CO)CO
• InChI: 1S/C8H19NO5/c10-3-1-9(2-4-11)8(5-12,6-13)7-14/h10-14H,1-7H2
• InChI key: OWMVSZAMULFTJU-UHFFFAOYSA-N

Description

Packaging

100 g in poly bottle

1 kg in poly bottle

10, 25 kg in poly drum

Application

Bis Tris is a commonly used biological buffer for very broad usage in biological systems such as diagnostics, electrophoresis and protein based bioprocessing. The pKa of Bis Tris is 6.36 which makes it a good candidate for biological-based formulations.

Bis Tris is used in a wide variety of biological applications including protein separation buffers, cell culture media, electrophoresis separations and diagnostic reagent formulations.

Safety Information

• Pictograms: GHS05
• Signal Word: Danger
• Hazard Statements: H318
• Precautionary Statements: P280 - P305 + P351 + P338 + P310
• Hazard Classifications: Eye Dam. 1
• Storage Class Code: 13 - Non Combustible Solids
• WGK: WGK 2
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable