Eye Irrit. 2 - Flam. Liq. 2
3 - Flammable liquids
57.2 °F - closed cup
14.0 °C - closed cup
If available for a given product, the recommended re-test date or the expiration date can be found on the Certificate of Analysis.
In general, slow loss of fluorescence has been observed with PKH1 and PKH2 in in vivo studies and it is likely that PKH67 may also slowly lose fluorescence. Leakage (or loss of staining intensity) may also occur due to cell-to-cell transfer of the dye. The most common cause of cell-to-cell dye transfer is inadequate washing of the cells. Wash cells 3-5 times after labeling and transfer samples to new tubes between washes.
Labeled cells that have been washed can be visualized in culture up to 100 days after staining (for non-dividing cells). The dye itself is stable and will divide equally when the cells divide. After staining with PKH dyes, you can observe as many as 8 divisions depending on how brightly the cells were stained initially and the amount of surface area on the cells. Most commonly, 4-6 divisions can be visualized.
The general labeling kit (Product No. PKH26GL) will label the membrane of the cells. For phagocytic cells, Product No. PKH26PCL is used. This kit utilizes dye aggregates for specific labeling of phagocytic cells in the presence of other cell types.
As long as the cell has an intact membrane, the PKH26 dye can label the cell.
Over-labeling of the cells will result in loss of membrane integrity and reduced cell recovery. Methods for improving cell viability can be found on the troubleshooting guide.
For Product PKH26GL, when using the concentrations indicated in the product bulletin, you can stain 30 samples (1x107 cells/sample).The kit provides 500 ul of PKH26 dye solution. You use 4 ul per sample. There is enough dye in the kit to stain 125 samples.The kit provides 60 mls of Diluent C. You use 2 mls per sample (1 ml for dilution of the dye, 1 ml for dilution of the cells). There is enough diluent for 30 samples.You can purchase additional diluent as Product CGLDILIf you need less dye, you can purchase Product MINI26 which contains 100 ul of dye and 10 mls of Diluent C. This kit contains enough dye to stain 25 samples and enough diluent to stain 5 samples.
We offer Product numbers:PKH2GL Both of these kits stain the cells with a dye that emits in the green fluorescent range.
A protocol for visualization of tissue sections can be found in the technical bulletin. For visualization of stained cells by immunofluorescence or flow cytometry, the cells can be fixed in 2% paraformaldehyde for 15 minutes. The use of other organic solvents will extract the dye from the cells. If internal labeling is desired, the cells can be permeabilized with saponin (50-75 μg/mL). Here is the link to the Troubleshooting Guide.
The lot specific COA document can be found by entering the lot number above under the "Documents" section.
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Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.
Ask a Scientist here.
PKH dyes are easy to use and achieve stable, uniform, and reproducible fluorescent labeling of live cells. PKH dyes are non-toxic membrane stains which produce high signal to noise ratio.
Lipophilic cell tracking dyes enable cancer biologists to track tumor and immune cell functions both in vitro and in vivo. Read the article to choose a right membrane dye kit for cell tracking and proliferation monitoring.
Optimal staining is a key component for studying tumorigenesis and progression. Learn useful tips and techniques for dye applications, including examples from recent studies.
A video about how you can use fluorescent cell tracking dyes in combination with flow and image cytometry to study interactions and fates of different cell types in vitro and in vivo.