MilliporeSigma
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PLA0199

Sigma-Aldrich

Rabbit anti-Vimentin Antibody, Affinity Purified

Powered by Bethyl Laboratories, Inc.

Synonym(s):
CTRCT30, HEL113, epididymis luminal protein 113
NACRES:
NA.41

biological source

rabbit

Quality Level

antibody form

affinity purified immunoglobulin

antibody product type

primary antibodies

grade

Powered by Bethyl Laboratories, Inc.

species reactivity

mouse, human

technique(s)

immunohistochemistry: 1:200-1:1,000
western blot: 1:2,000- 1:10,000

accession no.

NP_003371.2

shipped in

wet ice

storage temp.

2-8°C

Gene Information

rabbit ... Vimentin(7431)

General description

Vimentin is a specific type III intermediate filament (IF) protein. It is abundantly expressed in mesenchymal cells. The gene encoding this protein is localized on human chromosome 10p13.

Immunogen

The epitope recognized by PLA0199 maps to a region between residue 416 and 466 of human vimentin using the numbering given in entry NP_003371.2 (GeneID 7431).

Biochem/physiol Actions

Vimentin helps to preserve the structure of cell and stability of tissue in mesenchymal cells. It is involved in tumorigenesis, EMT (epithelial mesenchymal transition) and the metastatic spread of cancer. It also controls infectious internalization of human papillomavirus 16 pseudovirions.

Physical form

Tris-buffered Saline containing 0.1% BSA containing 0.09% Sodium Azide

Other Notes

Vimentin is an intermediate filament expressed mainly in mesenchymal tissue and in primitive (undifferentiated) non-mesenchymal cells. It is a Class III cytoplasmic filament that forms a dense cytoskeletal network that extends from the nucleus to the plasma membrane. Vimentin reorganization during mitosis is likely regulated via its multiple phosphorylation sites. Vimentin expression is also found in mesoderm derived epithelia. Immunohistochemistry (IHC) with anti-vimentin antibodies is often used to identify neoplasms of mesenchymal or mesodermal origin.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificate of Analysis

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Certificate of Origin

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