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PLA0238

Sigma-Aldrich

Rabbit anti-AKT1 Antibody, Affinity Purified

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Synonym(s):
AKT, AKT1m, CWS6, PKB, PKB alpha, PKB-ALPHA, PRKBA, Protein kinase B, RAC, RAC-ALPHA, RAC-PK-alpha, protein kinase B alpha, proto-oncogene c-Akt, rac protein kinase alpha, serine-threonine protein kinase, v-akt murine thymoma viral oncogene homolog 1, v-akt murine thymoma viral oncogene-like protein 1
NACRES:
NA.41

biological source

rabbit

Quality Level

antibody form

affinity purified immunoglobulin

antibody product type

primary antibodies

grade

Powered by Bethyl Laboratories, Inc.

species reactivity

human

technique(s)

immunohistochemistry: 1:500-1:2,000
western blot: 1:2,000- 1:10,000

accession no.

NP_005154.1

UniProt accession no.

shipped in

wet ice

storage temp.

2-8°C

Gene Information

rabbit ... AKT1(207)

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SAB4301414SAB5700675SAB5700129
antibody form

affinity purified immunoglobulin

antibody form

affinity isolated antibody

antibody form

affinity isolated antibody

antibody form

affinity isolated antibody

species reactivity

human

species reactivity

rat, human, mouse

species reactivity

human, mouse, rat

species reactivity

rat, human, mouse

technique(s)

immunohistochemistry: 1:500-1:2,000, western blot: 1:2,000- 1:10,000

technique(s)

western blot: 1:500-1:1000 (Cell Lysate)

technique(s)

immunofluorescence: 1:50-1:100, immunohistochemistry: 1:50-1:100, immunoprecipitation (IP): 1:50-1:200, western blot: 1:500-1:2000

technique(s)

western blot: 1:500-1:1000

UniProt accession no.

P31749

UniProt accession no.

P31749

UniProt accession no.

P31749

UniProt accession no.

P31749

shipped in

wet ice

shipped in

wet ice

shipped in

wet ice

shipped in

wet ice

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Immunogen

The epitope recognized by PLA0238 maps to a region between residue 25 and 75 of human v-akt murine thymoma viral oncogene homolog 1 using the numbering given in entry NP_005154.1 (GeneID 207).

Physical form

Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide

Other Notes

AKT1 is a serine-threonine kinase that functions downstream of PI-3 kinase and is known to phosphorylate several proteins. AKT1 mediates the effects of several growth factors such as insulin, IGF-1, EGF, and PDGF to control cell survival and apoptosis. There are numerous substrates of AKT1, some of which are: GSK3-alpha and beta, mTOR, TSC2, Bad, c-Raf, and caspase 9.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage Class Code

12 - Non Combustible Liquids

WGK

nwg

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable


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