PLN10

Sigma-Aldrich

GenElute Plasmid Miniprep Kit

greener alternative

sufficient for 10 purifications

Synonym(s):
Gen Elute, GenElute Plasmid Kit
NACRES:
NA.52
Pricing and availability is not currently available.

usage

sufficient for 10 purifications

Quality Level

greener alternative product characteristics

Designing Safer Chemicals
Learn more about the Principles of Green Chemistry.

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General description

The GenElute Plasmid Miniprep Kit offers a simple, rapid, and cost-effective method for isolating plasmid DNA from recombinant E. coli cultures. By combining silica-binding technology and the convenience of a spin column format, up to 15 μg of high copy plasmid DNA can be recovered from 1-5 mL of E. coli culture in less than 30 minutes. Note that actual yield and optimal volume of culture to use depend on the plasmid and the culture medium.

An overnight recombinant E. coli culture is harvested with centrifugation and subjected to a modified alkaline-SDS lysis procedure followed by adsorption of the DNA onto silica in the presence of high salts. Contaminants are then removed by a spin-wash step. Finally, the bound DNA is eluted in water or Tris-EDTA buffer.
We are committed to bringing you Greener Alternative Products, which adhere to one or more of The 12 Principles of Greener Chemistry. This product has Inherently Safer Chemistry, compared to the standard use of phenol and chloroform to perform DNA extractions.

Application

The DNA isolated with the help of this kit has been used for transfection.
The DNA is ready for immediate use in applications such as restriction enzyme digestion, cloning, PCR, transformation, transcription, conventional, and automated sequencing.

Features and Benefits

  • Purified plasmid DNA in less than 30 minutes
  • Purify up to 15 μg of plasmid DNA
  • No detectable genomic DNA or RNA contamination
  • No phenol/chloroform extraction or alcohol precipitation required

Other Notes

For additional information, please see www.sigma-aldrich.com/genelutehp.

Legal Information

GenElute is a trademark of Sigma-Aldrich Co. LLC

Pictograms

FlameExclamation mark

Signal Word

Warning

Hazard Statements

Precautionary Statements

RIDADR

UN 3316 9

Flash Point(F)

80.6 °F

Flash Point(C)

27 °C

Certificate of Analysis
Certificate of Origin
Effect of Differentially Expressed MicroRNAs 602 and 323-5p on Hepatitis C Virus Genotype 1b Viral Load in Infected Liver Cells
Samina Noorali
Journal of Infectious Diseases and Therapy (2014)
The influence of Nosema (Microspora: Nosematidae) infection on honey bee (Hymenoptera: Apidae) defense against Varroa destructor (Mesostigmata: Varroidae).
Bahreini R and Currie RW
Journal of Invertebrate Pathology, 57-65, 57-65 (2015)
Merijn L M Salverda et al.
PLoS genetics, 7(3), e1001321-e1001321 (2011-03-17)
Whether evolution is erratic due to random historical details, or is repeatedly directed along similar paths by certain constraints, remains unclear. Epistasis (i.e. non-additive interaction between mutations that affect fitness) is a mechanism that can contribute to both scenarios. Epistasis...
Aarti Kathrani et al.
PloS one, 7(1), e30117-e30117 (2012-01-27)
Single nucleotide polymorphisms (SNP) in the TLR5 gene have been associated with human inflammatory bowel disease (IBD) and animal models of this disease. We recently demonstrated a significant association between three non-synonymous SNPs in the canine TLR5 gene and IBD...
Isabella Monne et al.
Journal of clinical microbiology, 46(5), 1769-1773 (2008-03-28)
Among the different hemagglutinin (HA) subtypes of avian influenza (AI) viruses, H5, H7, and H9 are of major interest because of the serious consequences for the poultry industry and the increasing frequency of direct transmission of these viruses to humans....

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