Dirofilaria repens: emergence of autochthonous human infections in the Czech Republic (case reports).
BMC Infectious Diseases, 16, 171-171 (2016)
Ying Liu et al.
Bio-protocol, 8(5), e2753-e2753 (2018-03-05)
Cyclic di-guanosine monophosphate (c-di-GMP) is a ubiquitous second messenger that regulates distinct aspects of bacterial physiology. It is synthesized by diguanylate cyclases (DGCs) and hydrolyzed by phosphodiesterases (PDEs). To date, the activities of DGC and PDE are commonly assessed by...
Darin Bloemberg et al.
The CRISPR journal, 4(1), 104-119 (2021-02-23)
Since observations that CRISPR nucleases function in mammalian cells, many strategies have been devised to adapt them for genetic engineering. Here, we investigated self-cutting and integrating CRISPR-Cas9 plasmids (SCIPs) as easy-to-use gene editing tools that insert themselves at CRISPR-guided locations....
Simeon S Andrews et al.
The Journal of biological chemistry, 294(30), 11549-11558 (2019-06-12)
Two-hybrid systems can be used for investigating protein-protein interactions and may provide important information about gene products with unknown function. Despite their success in mapping protein interactions, two-hybrid systems have remained mostly untouched by improvements in next-generation DNA sequencing. The...
Robert Mans et al.
FEMS yeast research, 18(7) (2018-06-04)
Here, two methods are described for efficient genetic modification of Saccharomyces cerevisiae using CRISPR/Cas9. The first method enables the modification of a single genetic locus using in vivo assembly of a guide RNA (gRNA) expression plasmid without the need for...