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PLX15

Sigma-Aldrich

GenElute Plasmid Maxiprep Kit

greener alternative

sufficient for 15 purifications

Synonym(s):
GenElute Plasmid Kit, Gen Elute
NACRES:
NA.52

usage

sufficient for 15 purifications

Quality Level

greener alternative product characteristics

Designing Safer Chemicals
Learn more about the Principles of Green Chemistry.

greener alternative category

Aligned

storage temp.

15-25°C

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General description

The GenElute Plasmid Maxiprep Kit offers a simple, rapid, and cost-effective method for isolating plasmid DNA from recombinant E. coli cultures. By combining silica-binding technology and the convenience of a spin column format, up to 1.2 mg of plasmid DNA can be recovered from 25-200 ml of Luria Broth (LB) in about 45 minutes. Note that actual yield and optimal volume of culture to use depend on the plasmid and the culture medium.

An overnight recombinant E. coli culture is harvested by centrifugation and subjected to a modified alkaline-SDS lysis procedure followed by adsorption of the DNA onto silica in the presence of high salts. Contaminants are then removed by a spin-wash step. Finally, the bound DNA is eluted in water or Tris-EDTA buffer.

We are committed to bringing you Greener Alternative Products, which adhere to one or more of The 12 Principles of Greener Chemistry. This product has Inherently Safer Chemistry, compared to the standard use of phenol and chloroform to perform DNA extractions.

Application

The recovered plasmid DNA is predominantly in its supercoiled form. There is no visual evidence of genomic DNA or RNA contamination detected by agarose gel electrophoresis. The DNA is ready for immediate use in downstream applications such as restriction digestion, ligation, sequencing, PCR, and transfection.

Legal Information

GenElute is a trademark of Sigma-Aldrich Co. LLC

Signal Word

Danger

Hazard Classifications

Acute Tox. 4 Oral - Eye Irrit. 2 - Met. Corr. 1 - Resp. Sens. 1 - Skin Irrit. 2 - STOT SE 3

Target Organs

Central nervous system

Storage Class Code

8A - Combustible, corrosive hazardous materials

WGK

WGK 2

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificate of Analysis

Certificate of Origin

Han Liu et al.
FEBS letters, 583(14), 2365-2371 (2009-06-24)
The chaperonin GroEL contains two seven-subunit rings, and allosteric signals between them are required to complete the GroEL reaction cycle. For this reason SR1, a mutant of GroEL that forms only single rings, cannot function as a chaperone. Mutations in
Characterisation of mutations in GroES that allow GroEL to function as a single ring.
Liu H
Febs Letters, 583(14), 2365-2371 (2009)
Martina Herrmann et al.
Applied and environmental microbiology, 75(10), 3127-3136 (2009-03-24)
Communities of ammonia-oxidizing archaea (AOA) and bacteria (AOB) in freshwater sediments and those in association with the root system of the macrophyte species Littorella uniflora, Juncus bulbosus, and Myriophyllum alterniflorum were compared for seven oligotrophic to mesotrophic softwater lakes and

Protocols

Escort™ III Transfection Reagent Protocol

The product bulletin providin detailed use protocol for easy DNA transfection.

Escort™ IV Transfection Reagent Protocol

Product manual provides detailed protocol for easy DNA transfection.

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

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