PLX15

GenElute^™ Plasmid Maxiprep Kit

sufficient for 15 purifications

• Synonym(s): Gen Elute, GenElute^™ Plasmid Kit
• NACRES: NA.52
• UNSPSC Code: 41105501

Properties

• usage: sufficient for 15 purifications
• Quality Level: 200
• greener alternative product characteristics: Designing Safer Chemicals; Learn more about the Principles of Green Chemistry.
• sustainability: Greener Alternative Product
• technique(s): DNA purification: suitable
• test parameters: : ~45 min kit run time, sample volume: 25-200 mL bacterial culture
• greener alternative category: Aligned,
• storage temp.: 15-25°C

Description

General description

Click here to view the kit protocol

The GenElute Plasmid Maxiprep Kit offers a simple, rapid, and cost-effective method for isolating plasmid DNA from recombinant E. coli cultures. By combining silica-binding technology and the convenience of a spin column format, up to 1.2 mg of plasmid DNA can be recovered from 25-200 ml of Luria Broth (LB) in about 45 minutes. Note that actual yield and optimal volume of culture to use depend on the plasmid and the culture medium.

An overnight recombinant E. coli culture is harvested by centrifugation and subjected to a modified alkaline-SDS lysis procedure followed by adsorption of the DNA onto silica in the presence of high salts. Contaminants are then removed by a spin-wash step. Finally, the bound DNA is eluted in water or Tris-EDTA buffer.

We are committed to bringing you Greener Alternative Products, which adhere to one or more of The 12 Principles of Greener Chemistry. This product has Inherently Safer Chemistry, compared to the standard use of phenol and chloroform to perform DNA extractions.

Application

The recovered plasmid DNA is predominantly in its supercoiled form. There is no visual evidence of genomic DNA or RNA contamination detected by agarose gel electrophoresis. The DNA is ready for immediate use in downstream applications such as restriction digestion, ligation, sequencing, PCR, and transfection.

Legal Information

GenElute is a trademark of Sigma-Aldrich Co. LLC

Kit Components Also Available Separately

• L1912

  Lysis Solution

  SDS
• R6148

  RNase A solution

  SDS
• C2112

  Column Preparation Solution

  SDS
• R1149

  Resuspension Solution, Add RNase A before use. After addition of RNAse, store at 2-8ºC. )

  SDS

Safety Information

• pictograms: GHS08,GHS05,GHS07
• signalword: Danger
• hcodes: H290,H302,H315,H319,H334,H336
• pcodes: P234 - P261 - P264 - P301 + P312 - P302 + P352 - P305 + P351 + P338
• Hazard Classifications: Acute Tox. 4 Oral - Eye Irrit. 2 - Met. Corr. 1 - Resp. Sens. 1 - Skin Irrit. 2 - STOT SE 3
• target_organs: Central nervous system
• Storage Class: 10 - Combustible liquids
• flash_point_f: Not applicable
• flash_point_c: Not applicable
• wgk: WGK 3