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PPC1010

Sigma-Aldrich

Protease and Phosphatase Inhibitor Cocktail

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Synonym(s):
Protease and phosphatase inhibitor

form

DMSO solution

Quality Level

packaging

pkg of 1 ml, 5 ml ×

shipped in

wet ice

storage temp.

−20°C

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P1860P5726PIC0002
packaging

pkg of 1 ml, 5 ml ×

packaging

-

packaging

-

packaging

pkg of 1 mL (in amber polypropylene vial), pkg of 5 mL (in amber polypropylene vial)

shipped in

wet ice

shipped in

dry ice

shipped in

wet ice

shipped in

-

storage temp.

−20°C

storage temp.

−20°C

storage temp.

2-8°C

storage temp.

−20°C

Quality Level

200

Quality Level

200

Quality Level

300

Quality Level

-

General description

Protease and Phosphatase Inhibitor Cocktail us designed to increase the yield of intact and unmodified proteins in cell extracts. Protease and Phosphatase Inhibitor cocktail mixture inhibits serine, cysteine, and acid proteases, as well as aminopeptidases. Protease and Phosphatase Inhibitor Cocktail also inhibits serine/threonine protein phosphatases and L-isozymes of alkaline phosphatase. The cocktail has been tested on extracts from various animal tissues such as human placenta and bovine liver, and on extracts of A431, CHO, and U937 cells.

Protease and Phosphatase Inhibitor Cocktail is supplied as a ready-to-use solution in DMSO.

Application

Protease and Phosphatase Inhibitor Cocktail has been used in protein isolation buffers.

Preparation Note

The recommended dilution of the cocktail in the biological extract is 1 ml of the cocktail per 500 mg of protein extracted from tissue or cells. In many cases, the cocktail can be used at a final concentration of 1% (v/v), 1 ml of cocktail solution per 100 ml of extraction buffer.

Other Notes

For life science research only. Not for use in diagnostic procedures.

Pictograms

Exclamation mark

Signal Word

Warning

Hazard Statements

Hazard Classifications

Eye Irrit. 2 - Skin Irrit. 2

Storage Class Code

10 - Combustible liquids

WGK

WGK 2

Flash Point(F)

188.6 °F

Flash Point(C)

87 °C


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