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PPC1010

Sigma-Aldrich

Protease and Phosphatase Inhibitor Cocktail

DMSO solution, for the inhibition of serine, cysteine, acid proteases, aminopeptidase, serine/threonine protein phosphatases and L-isozymes of alkaline phosphatase

Synonym(s):

Protease and Phosphatase Inhibitor Mix, Protease and phosphatase inhibitor

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1 ML
$246.48
5 ML
$480.37

$246.48

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1 ML
$246.48
5 ML
$480.37

About This Item

UNSPSC Code:
12352200
NACRES:
NA.54
form:
DMSO solution
solubility:
water: soluble
storage temp.:
−20°C

$246.48

List Price$260.00Save 5%
Web-Only Promotion

In StockDetails


Product Name

Protease and Phosphatase Inhibitor Cocktail,

form

DMSO solution

Quality Level

packaging

pkg of 1 ml, 5 ml ×

solubility

water: soluble

shipped in

wet ice

storage temp.

−20°C

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This Item
P1860P8215P8340
form

DMSO solution

form

DMSO solution

form

DMSO solution

form

DMSO solution

Quality Level

200

Quality Level

200

Quality Level

300

Quality Level

300

storage temp.

−20°C

storage temp.

−20°C

storage temp.

−20°C

storage temp.

−20°C

packaging

pkg of 1 ml, 5 ml ×

packaging

-

packaging

-

packaging

-

shipped in

wet ice

shipped in

dry ice

shipped in

-

shipped in

-

General description

Protease and Phosphatase Inhibitor Cocktail is designed to increase the yield of intact and unmodified proteins in cell extracts. Protease and Phosphatase Inhibitor cocktail mixture inhibits serine, cysteine, and acid proteases, as well as aminopeptidase. It also inhibits serine/threonine protein phosphatases and L-isozymes of alkaline phosphatase. The cocktail has been tested on extracts from various animal tissues such as the human placenta and bovine liver and on extracts of A431, CHO, and U937 cells. Protease and Phosphatase Inhibitor Cocktail is supplied as a ready-to-use solution in DMSO.

Application

Protease and Phosphatase Inhibitor Cocktail has been used as a supplement in radioimmunoprecipitation assay (RIPA) buffer:
  • for cellular protein extraction[1]
  • to lyse purified CD4+ T cells[2]
  • to prepare protein extracts from placental tissues and cells[3]

Protease and Phosphatase Inhibitor Cocktail has been used in protein isolation buffers.[4][5]

Preparation Note

The recommended dilution of the cocktail in the biological extract is 1 ml of the cocktail per 500 mg of protein extracted from tissue or cells. In many cases, the cocktail can be used at a final concentration of 1% (v/v), 1 ml of cocktail solution per 100 ml of extraction buffer.

Other Notes

This product is for R&D use only, not for drug, household, or other uses. Please consult the Safety Data Sheet for information regarding hazards and safe handling practices.

pictograms

Exclamation mark

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Warning

hcodes

Hazard Classifications

Eye Irrit. 2 - Skin Irrit. 2

Storage Class

10 - Combustible liquids

wgk_germany

WGK 2

flash_point_f

188.6 °F

flash_point_c

87 °C


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Questions

1–2 of 2 Questions  
  1. Does PPC1010 contain EDTA?

    1 answer
    1. PPC1010 does not contain EDTA

      Helpful?

  2. Does these Protease and Phosphatase Inhibitor Cocktail contains any type of metal chelator or EDTA?

    1 answer
    1. This product does not contains EDTA or other chelators. See the link below to review the product datasheet which includes the cocktail components:
      https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/113/180/ppc1010dat.pdf

      Helpful?

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