PCR Plate Detection Kit

Pricing and availability is not currently available.


sufficient for 480 detection reactions

storage temp.



Perform solid-phase capture and sequence-specific detection of PCR products in an easy-to-use, automatable format and allele-specific hybridization for the detection and genotyping of point mutations. PCR amplification is carried out with one 5′-biotinylated primer and one unlabeled primer. The amplified products are immobilized in streptavidin-coated strip-well plates. The non-biotinylated strand is removed by sodium hydroxide denaturation, and the biotinylated strand is hybridized to a sequence-specific fluorescein-labeled probe. After a wash step, the probe is detected with a peroxidase-conjugated anti-fluorescein antibody and the chromogenic peroxidase substrate TMB. The assay can be completed in 2.5 hours.

Features and Benefits

  • Typically 10-100X more sensitive than gel electrophoresis
  • Discrimination between PCR product levels over a 2-2.5 log range of concentration
  • Protocol is easily scalable, allowing processing of eight to several hundred samples
  • Takes 2.5 hr total time (30-45 min hands-on); PlateHyb hybridization buffer reduces time required for efficient hybridization
  • Probe provides an additional level of sequence specificity; PlateHyb hybridization buffer improves genotyping of single base mutations

Legal Information

Purchase of this product is accompanied by a limited license for use in the Polymerase Chain Reaction (PCR) process for research purposes only and in conjunction with a thermal cycler whose use in the automated performance of the PCR process is covered by an up-front license fee, either by payment to Applied Biosystems or as purchased, i.e., and authorized thermal cycler.

Kit Components Also Available Separately

Product No.

  • Z369675Thermoplate covers 15 ea

  • T8665TMB Liquid Substrate System 2 x 100

  • P3563Wash buffer dry packs (phosphate buffered saline with Tween 20, pH 7.4) 10 ea


UN 2924 3/PG 3

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