Documents

PPD1

Sigma-Aldrich

PCR Plate Detection Kit

Pricing and availability is not currently available.

usage

sufficient for 480 detection reactions

storage temp.

2-8°C

Application

Perform solid-phase capture and sequence-specific detection of PCR products in an easy-to-use, automatable format and allele-specific hybridization for the detection and genotyping of point mutations. PCR amplification is carried out with one 5′-biotinylated primer and one unlabeled primer. The amplified products are immobilized in streptavidin-coated strip-well plates. The non-biotinylated strand is removed by sodium hydroxide denaturation, and the biotinylated strand is hybridized to a sequence-specific fluorescein-labeled probe. After a wash step, the probe is detected with a peroxidase-conjugated anti-fluorescein antibody and the chromogenic peroxidase substrate TMB. The assay can be completed in 2.5 hours.

Features and Benefits

  • Typically 10-100X more sensitive than gel electrophoresis
  • Discrimination between PCR product levels over a 2-2.5 log range of concentration
  • Protocol is easily scalable, allowing processing of eight to several hundred samples
  • Takes 2.5 hr total time (30-45 min hands-on); PlateHyb hybridization buffer reduces time required for efficient hybridization
  • Probe provides an additional level of sequence specificity; PlateHyb hybridization buffer improves genotyping of single base mutations

Legal Information

Purchase of this product is accompanied by a limited license for use in the Polymerase Chain Reaction (PCR) process for research purposes only and in conjunction with a thermal cycler whose use in the automated performance of the PCR process is covered by an up-front license fee, either by payment to Applied Biosystems or as purchased, i.e., and authorized thermal cycler.

Kit Components Also Available Separately

Product No.
Description
SDS

  • Z369675Thermoplate covers 15 ea

  • T8665TMB Liquid Substrate System 2 x 100

  • P3563Wash buffer dry packs (phosphate buffered saline with Tween 20, pH 7.4) 10 ea

RIDADR

UN 2924 3/PG 3

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service

Social Media

LinkedIn icon
Twitter icon
Facebook Icon
Instagram Icon

MilliporeSigma

Research. Development. Production.

We are a leading supplier to the global Life Science industry with solutions and services for research, biotechnology development and production, and pharmaceutical drug therapy development and production.

© 2021 Merck KGaA, Darmstadt, Germany and/or its affiliates. All Rights Reserved.

Reproduction of any materials from the site is strictly forbidden without permission.