MilliporeSigma
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PROBES

Custom qPCR Probes

Design and Order DNA, Locked Nucleic Acid, and BHQ Probes for qPCR

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Synonym(s):
BHQ, BHQ Probes, Black Hole Quencher, Locked Nucleic Acid probes, Probes, Real-Time PCR Probes, locked nucleic acid, qPCR assays, qPCR designs
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Custom Oligos & qPCR Probes Online Tools
Product Resources Title
Custom DNA and RNA Oligos & qPCR Probes Product Resources

Dual-Labeled Probes

  • Amounts: 1, 3, 5, and 10 OD
  • Purification: HPLC
  • Sequence Lengths: 15 - 40 bases
  • Modifications: Locked Nucleic Acid available
  • Dyes: Comprehensive offering of reporters and quenchers
  • Quality Control: 100% mass spectrometry
  • Format: Supplied dry in amber tubes
  • Learn more
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Molecular Beacons

  • Amounts: 1, 3, 5, and 10 OD
  • Purification: HPLC
  • Sequence Lengths: 15 - 40 bases
  • Modifications: Locked Nucleic Acid available
  • Dyes: Comprehensive offering of reporters and quenchers
  • Quality Control: 100% mass spectrometry
  • Format: Supplied dry in amber tubes
  • Learn more
Order

LightCycler® Probes

  • Amounts: 0.1, 0.25, 1.5, and 15 OD
  • Purification: HPLC
  • Sequence Lengths: 15 - 40 bases
  • Modifications: Locked Nucleic Acid available
  • Dyes: Probe 1 fluorescein (3′ end); Probe 2 phosphate (3′ end) and LC Red 610, 640, 670 or 705 (5′ end)
  • Quality Control: 100% mass spectrometry
  • Format: Supplied dry in amber tubes
  • Learn more
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Scorpions® Probes

  • Amounts: 1, 5, and 5 OD
  • Purification: HPLC
  • Sequence Lengths: 30 - 60 bases
  • Modifications: Locked Nucleic Acid available
  • Dyes: Comprehensive offering of reporters and quenchers
  • Quality Control: 100% mass spectrometry
  • Format: Supplied dry in amber tubes
  • Learn more
Order

Locked Nucleic Acid

  • Available for all Custom qPCR Probe Types
  • Increased thermal stability and hybridization specificity
  • Easier and more specific designs for problematic target sequences
  • Learn more
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Black Hole Quencher™ (BHQ™)

  • Available for all Custom qPCR Probe types
  • No native fluorescence (emits heat instead of light), resulting in lower background fluorescence
  • Increased signal-to-noise ratio, providing higher sensitivity
  • Enables a wider choice of reporters for multiplexing
  • Learn more
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Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

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