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PROBES

Custom qPCR Probes

Design and Order DNA, Locked Nucleic Acid, and BHQ Probes for qPCR

Synonym(s):
Black Hole Quencher, locked nucleic acid, qPCR designs, BHQ, Real-Time PCR Probes, Probes, BHQ Probes, qPCR assays, Locked Nucleic Acid probes


Dual-Labeled Probes

  • Amounts: 1, 3, 5, and 10 OD
  • Purification: HPLC
  • Sequence Lengths: 15 - 40 bases
  • Modifications: Locked Nucleic Acid available
  • Dyes: Comprehensive offering of reporters and quenchers
  • Quality Control: 100% mass spectrometry
  • Format: Supplied dry in amber tubes
  • Learn more

Molecular Beacons

  • Amounts: 1, 3, 5, and 10 OD
  • Purification: HPLC
  • Sequence Lengths: 15 - 40 bases
  • Modifications: Locked Nucleic Acid available
  • Dyes: Comprehensive offering of reporters and quenchers
  • Quality Control: 100% mass spectrometry
  • Format: Supplied dry in amber tubes
  • Learn more

LightCycler® Probes

  • Amounts: 0.1, 0.25, 1.5, and 15 OD
  • Purification: HPLC
  • Sequence Lengths: 15 - 40 bases
  • Modifications: Locked Nucleic Acid available
  • Dyes: Probe 1 fluorescein (3′ end); Probe 2 phosphate (3′ end) and LC Red 610, 640, 670 or 705 (5′ end)
  • Quality Control: 100% mass spectrometry
  • Format: Supplied dry in amber tubes
  • Learn more

Scorpions® Probes

  • Amounts: 1, 5, and 5 OD
  • Purification: HPLC
  • Sequence Lengths: 30 - 60 bases
  • Modifications: Locked Nucleic Acid available
  • Dyes: Comprehensive offering of reporters and quenchers
  • Quality Control: 100% mass spectrometry
  • Format: Supplied dry in amber tubes
  • Learn more

Locked Nucleic Acid

  • Available for all Custom qPCR Probe Types
  • Increased thermal stability and hybridization specificity
  • Easier and more specific designs for problematic target sequences
  • Learn more

Black Hole Quencher™ (BHQ™)

  • Available for all Custom qPCR Probe types
  • No native fluorescence (emits heat instead of light), resulting in lower background fluorescence
  • Increased signal-to-noise ratio, providing higher sensitivity
  • Enables a wider choice of reporters for multiplexing
  • Learn more

Articles

Blood Screening for Herpesviruses using qPCR

Hudnall, et al. demonstrates the use of easy and effective qPCR assys to screen blood for herpesvirus.

Molecular Beacons

Molecular Beacons are structured probes that are highly sensitive, sequence specific, and are used for sequence detection in qPCR and in vitro studies.

Dual-Labeled Probes in Digital PCR

Digital PCR (dPCR) is an end-point PCR method that is used for absolute quantification and for analysis of minority sequences against a background of similar majority sequences.

Onyx Quencher™ for Probed-Based qPCR

Onxy Quencher emits heat instead of light and offers and improved signal-to-noise ratio over fluorescent quenchers.

Related Content

LightCycler® Probes

LightCycler probes are highly sensitive and sequence-specific fluorescent probes designed for use with the Roche LightCycler instruments.

Custom Oligo Blanket Purchase Orders

We are pleased to offer a tracking system for your custom oligo Blanket Purchase Orders. Our tracking system provides easy management of expenditures against your Blanket Purchase Order (P.O.). Simply register your Blanket P.O. to take advantage of the FREE tracking.

OligoArchitect™ Consultative

For more complex and demanding applications, utilize our consultative service to ensure the success of your assay.

Oligonucleotide Label Licenses

Label licenses for our oligonucleotide products, including Black Hole Quencher®, BlackBerry® Quencher, and Locked Nucleic Acid (LNA®).

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Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

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