PROTTOT

Millipore

ProteoPrep® Total Extraction Sample Kit

NACRES:
NA.32

Quality Level

form

dry powder

storage temp.

2-8°C

General description

ProteoPrep® Total Extraction Sample Kit is designed to extract all proteins from any type of cell or tissue. It includes two reagents that have been used extensively for extraction and two new reagents with increased solubilizing power to extract more proteins. This kit also includes reagents for the reduction and alkylation of disulfide bonds. This allows comparison of the protein extractions obtained with each of the four reagents and optimization to meet your individual needs. The special reducing and alkylating reagents produce protein samples that exhibit improved focusing and decreased streaking in 2D gels.

Application

ProteoPrep® Total Extraction Sample Kit has been used to extract total protein from various sources such as epidermal stem cells and rheumatoid arthritis (RA) synovial fibroblasts.

Features and Benefits

  • Four pre-mixed solubilization reagents - Enables rapid solubilization.
  • Pre-measured reducing and alkylating reagents - Easy-to-use reagents provide improved IEF resolution.
  • Innovative detergent preparations - Highly improved solubility allows higher protein loads and greater visibility of low abundance proteins in 2D gels.

Storage and Stability

These reagents should remain stable for at least 6 months in their unopened container.

Other Notes

The ProteoPrep® Total Extraction Kit was designed through a collaboration of Proteome Systems and Sigma research scientists.
This kit contains enough reagents to generate at least ten 2-ml samples using all four reagents. For researchers who have optimized an extraction protocol using one chaotropic extraction reagent, each reagent is available as an individual product as well.

Legal Information

ProteoPrep is a registered trademark of Sigma-Aldrich Co. LLC

Disclaimer

Samples containing high amount of salts and buffers may not work with this kit. These samples should be dialyzed prior to use. This product is for R&D use only, not for drug, household, or other uses.

Kit Components Also Available Separately

Product No.
Description
SDS

  • C0481Protein Extraction Reagent Type 1

  • C0606Protein Extraction Reagent Type 2

  • C0731Protein Extraction Reagent Type 3

  • C0356Protein Extraction Reagent Type 4

  • A3221Iodoacetamide, Single use vial of 56 mg 5 x 56

  • T7567Tributylphosphine solution, 200 mM (in N-methyl-2-pyrrolidinone), liquid 5 x 0.5

Signal Word

Danger

hazcat

Acute Tox. 3 Oral - Aquatic Chronic 2 - Carc. 2 - Eye Dam. 1 - Repr. 1B - Self-heat. 1 - Skin Corr. 1B - Skin Sens. 1 - STOT SE 3

Target Organs

Respiratory system

storage_class_code

4.2 - Pyrophoric and self-heating hazardous materials

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificate of Analysis

Certificate of Origin

Suyang Yu et al.
International journal of molecular medicine, 41(4), 2185-2192 (2018-02-03)
Annexin A9 (ANXA9), a member of annexin family, has been reported be associated with colorectal cancer (CRC) carcinogenesis. However, the clinical significance of ANXA9 in CRC, particularly its correlation to invasion and metastasis remains ambiguous. The aim of the present study...
Glutamine with probiotics attenuates intestinal inflammation and oxidative stress in a rat burn injury model through altered iNOS gene aberrant methylation
Gong Zhen-Yu, et al.
American Journal of Translational Research (2017)
Selim Ceylan et al.
Canadian journal of microbiology, 57(4), 295-302 (2011-04-16)
A gram-negative, moderately halophilic bacterium was isolated from Çamaltı Saltern area, located in the Aegean Region of Turkey. Analysis of its 16S rRNA gene sequence and physiological characteristics showed that this strain belonged to the genus Halomonas ; hence, it...
Brucine inhibits TNF-a-induced HFLS-RA cell proliferation by activating the JNK signaling pathway
Tang M, et al.
Experimental and Therapeutic Medicine (2019)
Tsuyoshi Nakanishi et al.
Journal of biomolecular techniques : JBT, 18(5), 278-286 (2008-01-02)
We have developed a method for on-membrane direct identification of phosphoproteins, which are detected by a phosphate-binding tag (Phos-tag) that has an affinity to phosphate groups with a chelated Zn2+ ion. This rapid profiling approach for phosphoproteins combines chemical inkjet...

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service