PROTTWO

Sigma-Aldrich

ProteoPrep® Universal Extraction Kit

NACRES:
NA.32

Quality Level

form

dry powder

storage temp.

2-8°C

Application

This kit features new and innovative detergents, and uses specially formulated reagents and an optimized protocol designed to generate two prepared subcellular fractions that are uniquely ready for two-dimensional (2D) electrophoresis.
  • Fraction 1: Soluble/Cytoplasmic Proteins
  • Fraction 2: Membrane Proteins
The special reducing and alkylating reagents produce samples that exhibit improved focusing and decreased streaking in 2D gels. The ProteoPrep Universal Extraction Kit was designed through a collaboration of Proteome Systems and Sigma research scientists.

Features and Benefits

  • Innovative detergent preparations - Highly improved solubility allows for higher protein loads and greater visibility of low abundance proteins on 2D gels.
  • Two pre-mixed solubilizaton solutions - Generates two distinct populations for easy 2D analysis.
  • Pre-measured reducing and alkylating reagents - Easy-to-use reagents provide improved IEF resolution.
  • Pre-weighed dry blends - Stable and easy to reconstitute
  • Conveniently Packaged - No waste. Use only the amount needed.

Legal Information

ProteoPrep is a registered trademark of Sigma-Aldrich Co. LLC

Kit Components Also Available Separately

Product No.
Description
SDS

  • C0356Protein Extraction Reagent Type 4 23 mL

  • T7567Tributylphosphine solution, 200 mM (in N-methyl-2-pyrrolidinone), liquid 5 x 0.5

  • A3221Iodoacetamide, Single use vial of 56 mg 5 x 56

Signal Word

Danger

hazcat

Acute Tox. 3 Oral - Aquatic Chronic 2 - Carc. 2 - Eye Dam. 1 - Repr. 1B - Self-heat. 1 - Skin Corr. 1B - Skin Sens. 1 - STOT SE 3

Target Organs

Respiratory system

storage_class_code

4.2 - Pyrophoric and self-heating hazardous materials

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificate of Analysis

Certificate of Origin

Maria De Angelis et al.
Electrophoresis, 31(13), 2126-2136 (2010-07-02)
Food allergy is recognized as one of the major health concerns. It is estimated that ca. 4% of the population is affected by food allergenic disorders. Food allergies are defined as IgE-mediated hypersensitivity reactions. Foods such as peanuts, tree nuts...
Mustafa R Morsy et al.
Gene, 344, 171-180 (2005-01-20)
Rice (Oryza sativa L.) is sensitive to chilling particularly at early stages of seedling establishment. Two closely related genes (OsLti6a, OsLti6b), which are induced by low temperature during seedling emergence were isolated from a cold tolerant temperate japonica rice cultivar....
FcR? Presence in TCR Complex of Double-Negative T Cells Is Critical for Their Regulatory Function.
Thomson, C.W., et al.
Journal of Immunological Methods, 177, 2250-2257 (2006)
W M Freeman et al.
Neuroscience, 159(1), 183-195 (2009-01-13)
Declining cognitive performance is associated with increasing age, even in the absence of overt pathological processes. We and others have reported that declining cognitive performance is associated with age-related changes in brain glucose utilization, long-term potentiation and paired-pulse facilitation, protein...
Susanne K Pedersen et al.
Journal of proteome research, 2(3), 303-311 (2003-06-20)
Abundant and hydrophilic nonmembrane proteins with isoelectric points below pH 8 are the predominant proteins identified in most proteomics projects. In yeast, however, low-abundance proteins make up 80% of the predicted proteome, approximately 50% have pl's above pH 8 and...

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

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