MilliporeSigma
All Photos(1)

Documents

PURE1A

Sigma-Aldrich

Protein A Antibody Purification Kit

sufficient for 10 purifications

Sign Into View Organizational & Contract Pricing

NACRES:
NA.32

usage

sufficient for 10 purifications

shipped in

wet ice

storage temp.

2-8°C

General description

The Protein A Antibody Purification Kit provides all of the reagents necessary to isolate monoclonal and polyclonal antibodies.

Specificity

Capacity/Run (by Species)
Human IgG 20-25 mg
Mouse IgG1 8-12 mg
Mouse IgG2a 10-15 mg
Mouse IgG2b 6-10 mg
Mouse IgG3 15-20 mg
Rabbit IgG 8-12 mg
Goat IgG 2-4 mg
Bovine IgG 8-12 mg

Application

PURE-1A offers Protein A technology in a prepackaged, easy to use kit form. With this kit, milligram quantities of IgG can be purified from serum, ascites, or cell culture supernatants.
Purification of IgG from other species may be possible, however, the researcher will have to determine the suitability of the kit for their application.
The Protein A Antibody Purification Kit is suitable for use with IgGs from human, mouse, rabbit, goat, and bovine species.

Features and Benefits

  • High capacity - purify up to 8 mg of mouse IgG or 25 mg of human IgG per column run
  • Specific - will only bind IgG
  • Easy to use - antibody is eluted and desalted in a single step, ready to use
  • Gentle - avoids prolonged exposure of the antibody to low pH

Principle

Using our cartridge system, antibodies elute as highly purified proteins at physiological pH. Protein A is a powerful tool for isolation of antibodies from mammalian hosts. Protein A exhibits a high degree of specificity for IgG and ensures an antibody preparation virtually free of IgA, IgM and non-immunoglobulin serum proteins such as albumin.

Kit Components Only

Product No.
Description

  • Binding Buffer 225 mL

  • Desalting Cartridge 1 ea

  • Elution Buffer 75 mL

  • HEPES Buffer 225 mL

  • 5 ml Syringe 1

  • 10 ml Syringe 1

  • Protein A Cartridge 1 mL

  • Regeneration Buffer 75 mL

See All (8)

related product


Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

Already Own This Product?

Documents related to the products that you have purchased in the past have been gathered in the Document Library for your convenience.

Visit the Document Library

Difficulty Finding Your Product Or Lot/Batch Number?

Product numbers are combined with Pack Sizes/Quantity when displayed on the website (example: T1503-25G). Please make sure you enter ONLY the product number in the Product Number field (example: T1503).

Example:

T1503
Product Number
-
25G
Pack Size/Quantity

Additional examples:

705578-5MG-PW

PL860-CGA/SHF-1EA

MMYOMAG-74K-13

1000309185

enter as 1.000309185)

Having trouble? Feel free to contact Technical Service for assistance.

Lot and Batch Numbers can be found on a product's label following the words 'Lot' or 'Batch'.

Aldrich Products

  • For a lot number such as TO09019TO, enter it as 09019TO (without the first two letters 'TO').

  • For a lot number with a filling-code such as 05427ES-021, enter it as 05427ES (without the filling-code '-021').

  • For a lot number with a filling-code such as STBB0728K9, enter it as STBB0728 without the filling-code 'K9'.

Not Finding What You Are Looking For?

In some cases, a COA may not be available online. If your search was unable to find the COA you can request one.

Request COA

Customers Also Viewed

Slide 1 of 2

1 of 2

Andreas H Laustsen et al.
Toxicon : official journal of the International Society on Toxinology, 99, 23-35 (2015-03-17)
The venom proteome of the monocled cobra, Naja kaouthia, from Thailand, was characterized by RP-HPLC, SDS-PAGE, and MALDI-TOF-TOF analyses, yielding 38 different proteins that were either identified or assigned to families. Estimation of relative protein abundances revealed that venom is
Improved ELISA test for determination of potency of Inactivated Poliovirus Vaccine (IPV)
Hwang,G et al.
Sensors, 8(12), 2074-2079 (2008)
Ignacio Gallardo-Carreño et al.
Frontiers in microbiology, 11, 590736-590736 (2021-01-05)
Perchlorate anions are produced by chemical industries and are important contaminants in certain natural ecosystems. Perchlorate also occurs in some natural and uncontaminated environments such as the Atacama Desert, the high Arctic or the Antarctic Dry Valleys, and is especially
Penio T Todorov et al.
Cancer research, 67(23), 11419-11427 (2007-12-07)
Proteolysis-inducing factor (PIF) is a sulfated glycoprotein produced by cachexia-inducing tumors, which induces atrophy of skeletal muscle. PIF has been shown to bind specifically with high affinity (K(d), in nanomolar) to sarcolemma membranes from skeletal muscle of both the mouse
Marcella de Champdoré et al.
Analytical chemistry, 79(2), 751-757 (2007-01-16)
Patulin is a toxic secondary metabolite of a number of fungal species belonging to the genera Penicillum and Aspergillus. It has been mainly isolated from apples and apple products contaminated with the common storage-rot fungus of apples, Penicillum expansum, but

Protocols

Antibody purification protocols yield preparations containing endogenous IgG alongside specific antibodies.

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service