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QR0200

Sigma-Aldrich

Quantitative RT-PCR ReadyMix™

One step RT-qPCR for probe-based methods, MMLV & hot-start Taq

Synonym(s):

1-step RT-qPCR mix, Quantitative real-time PCR master mix

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$538.00

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About This Item

UNSPSC Code:
41106300
NACRES:
NA.55

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Quality Level

300

usage

sufficient for 250 reactions

feature

dNTPs included
hotstart

technique(s)

RT-qPCR: suitable

color

colorless

input

purified RNA

compatibility

ABI 5700
 ABI 7000
 ABI 7300
 ABI 7500 Fast
 ABI 7500
 ABI 7700
 ABI 7900 HT
ABI 7900 HT Fast
 ABI 7900
 ABI StepOne
 ABI StepOnePlus
 ABI ViiA 7
 Bio-Rad CFX384
 Bio-Rad CFX96
 Bio-Rad MJ Chromo4
 Bio-Rad MJ Opticon 2
 Bio-Rad MJ Opticon
 Bio-Rad MiniOpticon
 Bio-Rad MyiQ
 Bio-Rad iCycler iQ
 Bio-Rad iQ5
 Cepheid SmartCycler
 Eppendorf® Mastercycler ep realplex2 s
 Eppendorf® Mastercycler ep realplex
 Illumina Eco qPCR
 Qiagen Corbett Rotor-Gene 3000
 Qiagen Corbett Rotor-Gene 6000
 Qiagen Corbett Rotor-Gene Q
 Roche LightCycler 480
 Strategene Mx3000P
 Strategene Mx3005P
 Strategene Mx4000

detection method

probe-based

shipped in

wet ice

storage temp.

−20°C

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1 of 4

This Item
S9194P4600D7440
Quantitative RT-PCR ReadyMix™ One step RT-qPCR for probe-based methods, MMLV & hot-start Taq

Sigma-Aldrich

QR0200

Quantitative RT-PCR ReadyMix™

SYBR® Green JumpStart™ Taq ReadyMix™ for High Throughput qPCR SYBR® Green qPCR reagent, passive reference dye included

Sigma-Aldrich

S9194

SYBR® Green JumpStart™ Taq ReadyMix™ for High Throughput qPCR

ReadyMix™ Taq PCR Reaction Mix with MgCl2

Sigma-Aldrich

P4600

ReadyMix™ Taq PCR Reaction Mix

JumpStart™ Taq ReadyMix™ for Quantitative PCR For probe-based real-time PCR

Sigma-Aldrich

D7440

JumpStart™ Taq ReadyMix™ for Quantitative PCR

technique(s)

RT-qPCR: suitable

technique(s)

qPCR: suitable

technique(s)

PCR: suitable

technique(s)

qPCR: suitable

usage

sufficient for 250 reactions

usage

sufficient for 20 reactions, sufficient for 2000 reactions, sufficient for 400 reactions

usage

sufficient for 100 reactions

usage

sufficient for 100 reactions, sufficient for 20 reactions, sufficient for 400 reactions

feature

dNTPs included

feature

dNTPs included, hotstart

feature

dNTPs included, hotstart: no

feature

Multiplex PCR, dNTPs included, hotstart

color

colorless

color

colorless

color

colorless

color

colorless

input

purified RNA

input

purified DNA

input

purified DNA

input

purified DNA

compatibility

ABI 5700

compatibility

for use with ABI 5700, for use with ABI 7000, for use with ABI 7300, for use with ABI 7700, for use with ABI 7900 Fast, for use with ABI 7900 HT, for use with ABI StepOne, for use with ABI StepOnePlus

compatibility

-

compatibility

Bio-Rad MyiQ ( ), Bio-Rad iCycler iQ, Bio-Rad iQ 5, for use with ABI 5700, for use with ABI 7000, for use with ABI 7300, for use with ABI 7500 Fast, for use with ABI 7700, for use with ABI 7900 Fast, for use with ABI 7900 HT, for use with ABI StepOne, for use with ABI StepOnePlus, for use with ABI ViiA 7, for use with Bio-Rad CFX384, for use with Bio-Rad CFX96, for use with Bio-Rad MJ Chromo4, for use with Bio-Rad MJ Opticon 2, for use with Bio-Rad MJ Opticon Cepheid SmartCycler, for use with Bio-Rad MiniOpticon, for use with Eppendorf® Mastercycler ep realplex2 s, for use with Illumina Eco qPCR, for use with Qiagen Corbett Rotor-Gene 3000, for use with Qiagen Corbett Rotor-Gene 6000, for use with Qiagen Corbett Rotor-Gene Q, for use with Roche LightCycler 480, for use with Strategene Mx3000P, for use with Strategene Mx3005P, for use with Strategene Mx4000

General description

Quantitative RT-PCR ReadyMix™ combines Moloney Murine Leukemia Virus Reverse Transcriptase (M-MLV RT) and JumpStart™ Taq DNA polymerase in a one-step RT-PCR kit designed for the measurement of gene expression. This convenient 2X master mix includes M-MLV RT, JumpStart™ Taq DNA polymerase, 99% pure deoxynucleotides, buffer, glass passivator, and stabilizers. The JumpStart™ Taq DNA polymerase is an antibody-inactivated hot-start enzyme. Once the reaction temperature reaches 70°C, the DNA polymerase-antibody complex dissociates and Taq DNA polymerase activity is restored. This antibody-enzyme complex allows for easy and convenient set-up with less contamination risk than manual hot-start techniques. This kit has been formulated for fluorogenic hybridization probe-based detection methods.

Application

Quantitative RT-PCR ReadyMix™ has been used in the quantification of messenger RNA (mRNA) levels of specific expressed genes by quantitative reverse-transcription polymerase chain reaction (RT-qPCR).[1][2]

Features and Benefits

  • The master mix allows consistency and reproducibility from one reaction to the next
  • Reduced risk of contamination from multiple pipetting steps
  • Reduced set-up time as compared to manual or wax Hot Start methods
  • JumpStart™Taq Polymerase reduces primer-dimer and non-specific product formation
  • Broad instrument compatibility
  • Includes a separate ROX reference dye vial for reaction normalization

Packaging

1 kit sufficient for 250 reactions at 20 μL each or for 100 reactions at 50 μL each.

Legal Information

This product is for research use only.
Eppendorf is a registered trademark of Eppendorf AG
JumpStart is a trademark of Sigma-Aldrich Co. LLC
ReadyMix is a trademark of Sigma-Aldrich Co. LLC

Kit Components Only

Product No.
Description
  • Probe Based qRT-PCR ReadyMix 2 X
  • Moloney Murine Leukemia Viral Reverse Transcriptase (M-MLV RT) 5000 U

Kit Components Also Available Separately

Product No.
Description
SDS
  • P219210X PCR Buffer, Optimized for routine PCR with MgCl2 included 1.5 mL/vialSDS
  • M8787Magnesium chloride solution, PCR Reagent, 25 mM MgCI2 solution for PCR 25 mMSDS
  • P219210X PCR Buffer, Optimized for routine PCR with MgCl2 included 100 XSDS

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pictograms

Exclamation markEnvironment
GHS07,GHS09

signalword

Warning

Hazard Classifications

Aquatic Acute 1 - Aquatic Chronic 1 - Eye Irrit. 2 - Skin Irrit. 2 - Skin Sens. 1

Storage Class

12 - Non Combustible Liquids

flash_point_f

Not applicable

flash_point_c

Not applicable

Choose from one of the most recent versions:

Certificates of Analysis (COA)

Lot/Batch Number
SLCH0651
SLCG8790
SLCG9928
SLCG4184
SLCG4181

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Find documentation for the products that you have recently purchased in the Document Library.

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Validation of Quantitative PCR Assays
Lovatt, A., et al.
BioPharm., 22-32 (2002)
An Integrated High-Throughput System for mRNA Purification and Quantitation for Use in Identifying Gene Knockdown by RNA Interference
Wang, et al.
JALA: Journal of the Association for Laboratory Automation, 11, 314-318 (2006)
A screen of shRNAs targeting tumor suppressor genes to identify factors involved in A549 paclitaxel sensitivity
Ji D, et al.
Oncology Reports, 18(6), 1499-1505 (2007)
S A Bustin
Journal of molecular endocrinology, 29(1), 23-39 (2002-08-30)
The fluorescence-based real-time reverse transcription PCR (RT-PCR) is widely used for the quantification of steady-state mRNA levels and is a critical tool for basic research, molecular medicine and biotechnology. Assays are easy to perform, capable of high throughput, and can
T B Morrison et al.
BioTechniques, 24(6), 954-958 (1998-06-19)
Continuous fluorescence observation of amplifying DNA allows rapid and accurate quantification of initial transcript copy number. A simple and generic method for monitoring product synthesis with the double-stranded DNA dye, SYBR Green I provides initial template copy number estimation limited

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Protocols

Reverse Transcription Protocol (One-step Probe Detection)

Reverse transcription (RT) is the process of converting RNA to cDNA using a reverse transcriptase enzyme and dNTPs.

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