R0278

RIPA Buffer

• Synonym(s): Radioimmunoprecipitation assay buffer
• MDL number: MFCD02100484
• NACRES: NA.56

Properties

• description: protease free
• Quality Level: 200
• form: liquid
• packaging: pkg of 50 mL; pkg of 500 mL
• technique(s): immunoprecipitation (IP): suitable
• color: colorless
• pH: 7.8-8.2 (25 °C, 1 ×)
• storage temp.: 2-8°C

Description

General description

RIPA (Radio-Immunoprecipitation Assay) Buffer is supplied as a ready to use solution that requires no preparation. It minimizes non-specific protein-binding interactions to keep background low, while allowing most specific interactions to occur, enabling studies of relevant protein-protein interactions. It is widely used in applications such as immunoprecipitation since most antibodies and protein antigens are not adversely affected by the components of this buffer.

Radioimmunoprecipitation assay buffer (RIPA buffer) is a lysis buffer used for rapid, efficient cell lysis and solubilization of proteins from both adherent and suspension cultured mammalian cells.

Application

RIPA Buffer has been used as a reagent for cell lysis in the following experimental studies:

• Fibroblast-based deep phenotyping of the peripheral tissue, which facilitates the mechanistic disease stratification in sporadic Parkinson′s disease (sPD).
• Demonstrating the inhibition of oxidative phosphorylation (OXPHOS), which can activate the mixed lineage kinase domain-like protein (MLKL)-dependent necroptosis in human lung epithelial cells.

RIPA Buffer enables efficient cell lysis and protein solubilization while avoiding protein degradation and interference with the proteins′ immunoreactivity and biological activity. RIPA Buffer also results in low background in immunoprecipitation and molecular pull-down assays. Compatible with EZview^™ Affinity Gels.

Features and Benefits

1 mL of the RIPA Buffer is used to lyse cells from one 100 mm culture dish (0.5 to 5 * 107 cells) of most adherent mammalian cell lines.

Minimizes non-specific protein binding interactions to ensure low background.

Other Notes

Ready-to-use solution containing 150 mM NaCl, 1.0% IGEPAL^® CA-630, 0.5% sodium deoxycholate, 0.1% SDS, 50 mM Tris, pH 8.0.

Legal Information

EZview is a trademark of Sigma-Aldrich Co. LLC

IGEPAL is a registered trademark of Solvay