Sal I from Streptomyces albus G

Restriction Enzyme

CAS Number:
Enzyme Commission number:
MDL number:


for molecular biology


buffered aqueous glycerol solution


10,000 units/mL

shipped in

wet ice

storage temp.


Looking for similar products? Visit Product Comparison Guide


Recognition sequence: 5′-G/TCGAC-3′
Cutting results: a 2-10-fold Sal I overdigestion of 1 μg λ DNA substrate results in 100% cutting
Heat inactivation: Inactivated at 65 °C for 15 minutes.


SalI is a restriction endonuclease used for molecular biology methods to cleave DNA at the recognition sequence 5′-G/TCGAC-3′, generating fragments with 5′-cohesive ends.

Other Notes

Supplied with 10x Restriction Enzyme Buffer SH (B3657)

Unit Definition

One unit is the enzyme activity that completely cleaves 1 mg λDNA in 1 hr. at 37 °C in a total volume of 25 ml of buffer SH for restriction enzymes.

Physical form

Solution in 10 mM Tris-HCl, pH 7.5, 1 mM EDTA, 50% glycerol (v/v), 10 mM dithioerythritol, at 4 °C


NONH for all modes of transport

WGK Germany


In situ preservation of DNA in plant species.
Pyle, M.M., and Adams, R.P.
Taxon, 38, 576-576 (1989)
Y Katayama et al.
Antimicrobial agents and chemotherapy, 44(6), 1549-1555 (2000-05-19)
We have previously shown that the methicillin-resistance gene mecA of Staphylococcus aureus strain N315 is localized within a large (52-kb) DNA cassette (designated the staphylococcal cassette chromosome mec [SCCmec]) inserted in the chromosome. By sequence determination of the entire DNA...
A new restriction endonuclease from Streptomyces albus G.
J R Arrand et al.
Journal of molecular biology, 118(1), 127-135 (1978-01-05)
C Kessler et al.
Gene, 92(1-2), 1-248 (1990-08-16)
The properties and sources of all known class-I, class-II and class-III restriction endonucleases (ENases) and DNA modification methyltransferases (MTases) are listed and newly subclassified according to their sequence specificity. In addition, the enzymes are distinguished in a novel manner according...
Nico Mitro et al.
Methods in molecular biology (Clifton, N.J.), 952, 137-144 (2012-10-27)
The role of certain amino acids in the interactions of ligands with their cognate nuclear receptors is usually achieved by the resolution of the crystal structure of the receptor complexed with the ligand. As a complementary functional approach, site-directed mutagenesis...

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service

Social Media

LinkedIn icon
Twitter icon
Facebook Icon
Instagram Icon


Research. Development. Production.

We are a leading supplier to the global Life Science industry with solutions and services for research, biotechnology development and production, and pharmaceutical drug therapy development and production.

© 2021 Merck KGaA, Darmstadt, Germany and/or its affiliates. All Rights Reserved.

Reproduction of any materials from the site is strictly forbidden without permission.