R0754

Sigma-Aldrich

Sal I from Streptomyces albus G

Restriction Enzyme

CAS Number:
Enzyme Commission number:
MDL number:

grade

for molecular biology

form

buffered aqueous glycerol solution

concentration

10,000 units/mL

shipped in

wet ice

storage temp.

−20°C

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Specificity

Recognition sequence: 5′-G/TCGAC-3′
Cutting results: a 2-10-fold Sal I overdigestion of 1 μg λ DNA substrate results in 100% cutting
Heat inactivation: Inactivated at 65 °C for 15 minutes.

Application

SalI is a restriction endonuclease used for molecular biology methods to cleave DNA at the recognition sequence 5′-G/TCGAC-3′, generating fragments with 5′-cohesive ends.

Other Notes

Supplied with 10x Restriction Enzyme Buffer SH (B3657)

Unit Definition

One unit is the enzyme activity that completely cleaves 1 mg λDNA in 1 hr. at 37 °C in a total volume of 25 ml of buffer SH for restriction enzymes.

Physical form

Solution in 10 mM Tris-HCl, pH 7.5, 1 mM EDTA, 50% glycerol (v/v), 10 mM dithioerythritol, at 4 °C

RIDADR

NONH for all modes of transport

WGK Germany

3

Certificate of Analysis

Certificate of Origin

In situ preservation of DNA in plant species.
Pyle, M.M., and Adams, R.P.
Taxon, 38, 576-576 (1989)
Y Katayama et al.
Antimicrobial agents and chemotherapy, 44(6), 1549-1555 (2000-05-19)
We have previously shown that the methicillin-resistance gene mecA of Staphylococcus aureus strain N315 is localized within a large (52-kb) DNA cassette (designated the staphylococcal cassette chromosome mec [SCCmec]) inserted in the chromosome. By sequence determination of the entire DNA...
A new restriction endonuclease from Streptomyces albus G.
J R Arrand et al.
Journal of molecular biology, 118(1), 127-135 (1978-01-05)
Jaroslav Jelinek et al.
Epigenetics, 7(12), 1368-1378 (2012-10-19)
Genome wide analysis of DNA methylation provides important information in a variety of diseases, including cancer. Here, we describe a simple method, Digital Restriction Enzyme Analysis of Methylation (DREAM), based on next generation sequencing analysis of methylation-specific signatures created by...
Saqib H Ansari et al.
Journal of pediatric hematology/oncology, 35(4), e153-e156 (2013-02-08)
β-thalassemia is characterized by impaired β-chain synthesis leading to ineffective erythropoiesis, severe anemia, and a need for blood transfusion. Presence of Xmn I polymorphism (-158 C-T nucleotide change) in γ-globin gene is associated with a higher fetal hemoglobin and a...

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