R1145

RPMI-1640 Medium

Without ʟ-glutamine, folic acid and sodium bicarbonate, liquid, sterile-filtered, suitable for cell culture, 10x

• Synonym(s): RPMI Medium, RPMI-1640 Culture Medium, Roswell Park Memorial Institute 1640 medium
• UNSPSC Code: 12352207
• NACRES: NA.75
• MDL number: MFCD00217820

Properties

• Product Name: RPMI-1640 Medium, 10 ×, Without L-glutamine, folic acid and sodium bicarbonate, liquid, sterile-filtered, suitable for cell culture
• Quality Segment: 400
• sterility: sterile-filtered
• form: liquid
• concentration: 10 ×
• technique(s): cell culture | mammalian: suitable
• impurities: endotoxin, tested
• components: NaHCO₃: no; phenol red: yes; sodium pyruvate: no; HEPES: no; L-glutamine: no
• shipped in: ambient
• storage temp.: 2-8°C

Description

General description

RPMI 1640 Medium was developed at Roswell Park Memorial Institute in 1966 by Moore and his co-workers. A modification of McCoy′s 5A Medium, it was formulated to support lymphoblastoid cells in suspension culture, but it has since been shown to support a wide variety of cells that are anchorage-dependent. Originally intended to be used with a serum supplement, RPMI 1640 has been shown to support several cell lines in the absence of serum. It has also been widely used in fusion protocols and in the growth of hybrid cells. This medium is suitable for culturing human normal and neoplastic leukocytes.

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Preparation Note

Supplement with 0.3 g/L L-glutamine, 2.0 g/L sodium bicarbonate, 0.001 g/L folic acid at 1×.

Safety Information

• pictograms: GHS07
• signalword: Warning
• hcodes: H319
• pcodes: P264 - P280 - P305 + P351 + P338 - P337 + P313
• Hazard Classifications: Eye Irrit. 2
• Storage Class: 10 - Combustible liquids
• wgk: WGK 2
• flash_point_f: Not applicable
• flash_point_c: Not applicable