Skip to Content
MilliporeSigma
All Photos(1)

Documents

R1145

SAFC

RPMI-1640 Medium

Without ʟ-glutamine, folic acid and sodium bicarbonate, sterile liquid, suitable for cell culture, 10x

Pharma Manufacturing

Synonym(s):

RPMI Medium, RPMI-1640 Culture Medium, Roswell Park Memorial Institute 1640 medium

Sign Into View Organizational & Contract Pricing


About This Item

MDL number:
UNSPSC Code:
12352207
NACRES:
NA.75

product name

RPMI-1640 Medium, 10 ×, Without L-glutamine, folic acid and sodium bicarbonate, liquid, sterile-filtered, suitable for cell culture

Quality Level

sterility

sterile-filtered

form

liquid

concentration

10 ×

technique(s)

cell culture | mammalian: suitable

impurities

endotoxin, tested

components

NaHCO3: no
phenol red: yes
sodium pyruvate: no
HEPES: no
L-glutamine: no

shipped in

ambient

storage temp.

2-8°C

Looking for similar products? Visit Product Comparison Guide

General description

RPMI 1640 Medium was developed at Roswell Park Memorial Institute in 1966 by Moore and his co-workers. A modification of McCoy′s 5A Medium, it was formulated to support lymphoblastoid cells in suspension culture, but it has since been shown to support a wide variety of cells that are anchorage-dependent. Originally intended to be used with a serum supplement, RPMI 1640 has been shown to support several cell lines in the absence of serum. It has also been widely used in fusion protocols and in the growth of hybrid cells. This medium is suitable for culturing human normal and neoplastic leukocytes.

Reconstitution

Supplement with 0.3 g/L L-glutamine, 2.0 g/L sodium bicarbonate, 0.001 g/L folic acid at 1×.

pictograms

Exclamation mark

signalword

Warning

hcodes

Hazard Classifications

Eye Irrit. 2

wgk_germany

WGK 2

flash_point_f

Not applicable

flash_point_c

Not applicable


Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

Already Own This Product?

Find documentation for the products that you have recently purchased in the Document Library.

Visit the Document Library

Customers Also Viewed

Slide 1 of 8

1 of 8

Sigma-Aldrich

Sigma-Aldrich

R8755

RPMI-1640 Medium

Sigma-Aldrich

Sigma-Aldrich

R7513

RPMI-1640 Medium

Sigma-Aldrich

Sigma-Aldrich

R7388

RPMI-1640 Medium

RPMI-1640 Medium Modified, with sodium bicarbonate, without L-glutamine and phenol red, liquid, sterile-filtered, suitable for cell culture

Sigma-Aldrich

R7509

RPMI-1640 Medium

SAFC

SAFC

R5886

RPMI-1640 Medium

SAFC

SAFC

R4130

RPMI-1640 Medium

Folic acid BioReagent, suitable for cell culture, suitable for insect cell culture, suitable for plant cell culture, ≥97%

Sigma-Aldrich

F8758

Folic acid

Ryoichi Arita et al.
Diabetes, 58(1), 215-226 (2008-10-09)
Leukocyte adhesion in retinal microvasuculature substantially contributes to diabetic retinopathy. Involvement of the Rho/Rho kinase (ROCK) pathway in diabetic microvasculopathy and therapeutic potential of fasudil, a selective ROCK inhibitor, are investigated. Localization of RhoA/ROCK and Rho activity were examined in
Fhernanda R Smiderle et al.
BMC complementary and alternative medicine, 11, 58-58 (2011-07-27)
Mushroom polysaccharides have traditionally been used for the prevention and treatment of a multitude of disorders like infectious illnesses, cancers and various autoimmune diseases. Crude mushroom extracts have been tested without detailed chemical analyses of its polysaccharide content. For the
Tamara Veiga-Parga et al.
PLoS pathogens, 7(12), e1002427-e1002427 (2011-12-17)
Ocular herpes simplex virus infection can cause a blinding CD4⁺ T cell orchestrated immuno-inflammatory lesion in the cornea called Stromal Keratitis (SK). A key to controlling the severity of SK lesions is to suppress the activity of T cells that
Yoichi Matsuo et al.
International journal of cancer, 124(4), 853-861 (2008-11-28)
CXC-chemokines are involved in the chemotaxis of neutrophils, lymphocytes and monocytes. However, role of these chemokines in tumorigenesis, especially with regard to interaction between tumor and its microenvironment, has not been clearly elucidated. The purpose of this study was to
Ahmed Mosa et al.
Infection and immunity, 77(7), 2908-2918 (2009-04-29)
We analyzed the defensive role of the cytosolic innate recognition receptor nucleotide oligomerization domain 1 (NOD1) during infection with Listeria monocytogenes. Mice lacking NOD1 showed increased susceptibility to systemic intraperitoneal and intravenous infection with high or low doses of L.

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service