MilliporeSigma
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R1383

Sigma-Aldrich

RPMI-1640 Medium

With L-glutamine, without glucose and sodium bicarbonate, powder, suitable for cell culture

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Synonym(s):
Roswell Park Memorial Institute 1640 medium
MDL number:
MFCD00217820
NACRES:
NA.75

sterility

sterile

form

powder

technique(s)

cell culture | mammalian: suitable

components

HEPES: no
phenol red: yes
NaHCO3: no
sodium pyruvate: no
L-glutamine: yes

shipped in

ambient

storage temp.

2-8°C

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Application

RPMI 1640 Medium was developed at Roswell Park Memorial Institute in 1966 by Moore and his co-workers. A modification of McCoy′s 5A Medium, it was formulated to support lymphoblastoid cells in suspension culture, but it has since been shown to support a wide variety of cells that are anchorage-dependent. Originally intended to be used with a serum supplement, RPMI 1640 has been shown to support several cell lines in the absence of serum. It has also been widely used in fusion protocols and in the growth of hybrid cells. This medium is suitable for culturing human normal and neoplastic leukocytes.

Quantity

Formulated to contain 8.4 grams of powder per liter of medium.

Reconstitution

Supplement with 2.0 g/L sodium bicarbonate.

Storage Class Code

13 - Non Combustible Solids

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificate of Analysis

Enter Lot Number to search for Certificate of Analysis (COA).

Certificate of Origin

Enter Lot Number to search for Certificate of Origin (COO).

Product Information Sheet

Product Information Sheet - R1383

Formulation

RPMI-1640 Media

Quotes and Ordering

Bulk Quote-Order Product
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Fatty acids have known antifungal effects and are used in over-the-counter topical treatments. Screening of a collection of gene knockouts in Candida albicans revealed that one strain, carrying a deletion of the transcription factor DAL81, is very susceptible to the
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Precise acid-base homeostasis is essential for maintaining normal cell proliferation and growth. Conversely, dysregulated acid-base homeostasis, with increased acid extrusion and marked extracellular acidification, is an enabling feature of solid tumors, yet the mechanisms through which intra- and extracellular pH
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The Journal of experimental medicine, 215(1), 51-62 (2017-12-02)
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Protocols

Powdered Media Preparation Instructions

Powdered media and salt mixtures are extremely hygroscopic and should be protected from atmospheric moisture.

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