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RPMI-1640 Medium

With L-glutamine, without glucose and sodium bicarbonate, powder, suitable for cell culture

MDL number:






cell culture | mammalian: suitable


phenol red: yes
NaHCO3: no
sodium pyruvate: no
L-glutamine: yes

shipped in


storage temp.


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RPMI 1640 Medium was developed at Roswell Park Memorial Institute in 1966 by Moore and his co-workers. A modification of McCoy′s 5A Medium, it was formulated to support lymphoblastoid cells in suspension culture, but it has since been shown to support a wide variety of cells that are anchorage dependent. Originally intended to be used with a serum supplement, RPMI 1640 has been shown to support several cell lines in the absence of serum. It has also been widely used in fusion protocols and in the growth of hybrid cells.


Formulated to contain 8.4 grams of powder per liter of medium.


Supplement with 2.0 g/L sodium bicarbonate.

Storage Class Code

13 - Non Combustible Solids



Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificate of Analysis

Certificate of Origin

Jiayi Yao et al.
Cancers, 12(8) (2020-08-09)
The acidic pH of the tumor microenvironment plays a critical role in driving cancer development toward a more aggressive phenotype, but the underlying mechanisms are unclear. To this end, phenotypic and genotypic changes induced by adaptation of cancer cells to...
Qinxi Ma et al.
mSphere, 4(3) (2019-06-28)
Fatty acids have known antifungal effects and are used in over-the-counter topical treatments. Screening of a collection of gene knockouts in Candida albicans revealed that one strain, carrying a deletion of the transcription factor DAL81, is very susceptible to the...
Mette Flinck et al.
Cell cycle (Georgetown, Tex.), 17(9), 1056-1067 (2018-06-14)
Precise acid-base homeostasis is essential for maintaining normal cell proliferation and growth. Conversely, dysregulated acid-base homeostasis, with increased acid extrusion and marked extracellular acidification, is an enabling feature of solid tumors, yet the mechanisms through which intra- and extracellular pH...
Mark Y Jeng et al.
The Journal of experimental medicine, 215(1), 51-62 (2017-12-02)
The expansion of CD8+CD28- T cells, a population of terminally differentiated memory T cells, is one of the most consistent immunological changes in humans during aging. CD8+CD28- T cells are highly cytotoxic, and their frequency is linked to many age-related...
Martin Kolev et al.
Immunity, 52(3), 513-527 (2020-03-19)
Intrinsic complement C3 activity is integral to human T helper type 1 (Th1) and cytotoxic T cell responses. Increased or decreased intracellular C3 results in autoimmunity and infections, respectively. The mechanisms regulating intracellular C3 expression remain undefined. We identified complement, including...


Powdered Media Preparation Instructions

Powdered media and salt mixtures are extremely hygroscopic and should be protected from atmospheric moisture.

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