Cfo I from Clostridium formicoaceticum

Restriction Enzyme

CAS Number:
Enzyme Commission number:
MDL number:


for molecular biology


buffered aqueous glycerol solution


10,000 units/mL

shipped in

wet ice

storage temp.



Recognition Sequence: 5′-GCG/C-3′
Cutting results: a 2-10-fold Cfo I overdigestion of 1 μg λ DNA substrate results in 100% cutting
Heat inactivation: Not complete at 65 °C for 10 minutes.


CfoI is restriction endonuclease used in molecular biology methods to cleave DNA at the recognition site 5′-GCG/C-3′ to generate restriction fragments with 3′- cohesive ends.

Other Notes

Supplied with 10x Restriction Enzyme Buffer SL (B3782).


Isoschizomer: Hha I

Physical form

Solution in 20 mM Tris-HCl, pH 7.5, 1 mM EDTA, 100 mM NaCl, 10 mM 2-mercaptoethanol, 0.02% polydocanol, 0.1mM PMSF, 50% glycerol (v/v), at 4 °C


NONH for all modes of transport

WGK Germany


Certificate of Analysis
Certificate of Origin
R A Makula et al.
Nucleic acids research, 8(14), 3125-3131 (1980-07-25)
The purification and characterization of a new restriction endonuclease, Dde 1, from a sulfate-reducing, anaerobic bacterium, Desulfovibrio desulfuricans, Norway, is reported. The enzyme recognizes the sequence (see formula index) and cleaves at the position indicated by the arrows. The enzyme...
Alessia Ongaro et al.
Haematologica, 94(10), 1391-1398 (2009-08-04)
The antifolate agent methotrexate is an important component of maintenance therapy in acute lymphoblastic leukemia, although methotrexate-related toxicity is often a reason for interruption of chemotherapy. Prediction of toxicity is difficult because of inter-individual variability susceptibility to antileukemic agents. Methotrexate...
Vanja Karamatic Crew et al.
Transfusion, 47(3), 492-498 (2007-02-27)
The null phenotype of the Lutheran blood group system, Lu(null) or Lu(a-b-), is characterized by the lack of all Lutheran system antigens. It can arise from three genetic backgrounds: recessive, dominant, or X-linked. Lu(null) of the recessive type appears to...
Saqib H Ansari et al.
Journal of pediatric hematology/oncology, 35(4), e153-e156 (2013-02-08)
β-thalassemia is characterized by impaired β-chain synthesis leading to ineffective erythropoiesis, severe anemia, and a need for blood transfusion. Presence of Xmn I polymorphism (-158 C-T nucleotide change) in γ-globin gene is associated with a higher fetal hemoglobin and a...
Jaroslav Jelinek et al.
Epigenetics, 7(12), 1368-1378 (2012-10-19)
Genome wide analysis of DNA methylation provides important information in a variety of diseases, including cancer. Here, we describe a simple method, Digital Restriction Enzyme Analysis of Methylation (DREAM), based on next generation sequencing analysis of methylation-specific signatures created by...

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