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R2405

Sigma-Aldrich

StableCell RPMI-1640

With stable glutamine and sodium bicarbonate, liquid, sterile-filtered, suitable for cell culture

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Synonym(s):
RPMI-1640 Medium, Roswell Park Memorial Institute 1640 medium
MDL number:
NACRES:
NA.75

Quality Level

sterility

sterile-filtered

form

liquid

technique(s)

cell culture | mammalian: suitable

impurities

endotoxin, tested

components

sodium pyruvate: no
NaHCO3: yes
phenol red: yes
stable glutamine: yes
HEPES: no

shipped in

ambient

storage temp.

2-8°C

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This Item
R0883R7638R7509
Sigma-Aldrich

R2405

StableCell RPMI-1640

Sigma-Aldrich

R0883

RPMI-1640 Medium

Sigma-Aldrich

R7638

RPMI-1640 Medium

RPMI-1640 Medium Modified, with sodium bicarbonate, without L-glutamine and phenol red, liquid, sterile-filtered, suitable for cell culture

R7509

RPMI-1640 Medium

technique(s)

cell culture | mammalian: suitable

technique(s)

cell culture | mammalian: suitable

technique(s)

cell culture | mammalian: suitable

technique(s)

cell culture | mammalian: suitable

sterility

sterile-filtered

sterility

sterile-filtered

sterility

sterile-filtered

sterility

sterile-filtered

components

sodium pyruvate: no
NaHCO3: yes
phenol red: yes
stable glutamine: yes
HEPES: no

components

sodium pyruvate: no
phenol red: yes
L-glutamine: no
NaHCO3: yes
HEPES: no

components

sodium pyruvate: no
HEPES: 20 mM
L-glutamine: no
NaHCO3: yes
phenol red: yes

components

phenol red: no
L-glutamine: no
HEPES: no
sodium pyruvate: no
NaHCO3: yes

shipped in

ambient

shipped in

ambient

shipped in

ambient

shipped in

ambient

storage temp.

2-8°C

storage temp.

2-8°C

storage temp.

2-8°C

storage temp.

2-8°C

General description

RPMI 1640 Medium was developed at Roswell Park Memorial Institute in 1966 by Moore and his co-workers. A modification of McCoy′s 5A Medium, it was formulated to support lymphoblastoid cells in suspension culture, but it has since been shown to support a wide variety of cells that are anchorage-dependent. Originally intended to be used with a serum supplement, RPMI 1640 has been shown to support several cell lines in the absence of serum. It has also been widely used in fusion protocols and in the growth of hybrid cells. This medium is suitable for culturing human normal and neoplastic leukocytes.

Other Notes

This RPMI-1640 medium is supplemented with L-Ala-L-Gln dipeptide. This provides a more stable form of glutamine for cell culture. Free amino acid L-glutamine is known to be unstable in cell culture.

Legal Information

StableCell is a trademark of Sigma-Aldrich Co. LLC

Storage Class

12 - Non Combustible Liquids

wgk_germany

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)


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