R2756
EcoR V from Escherichia coli
buffered aqueous glycerol solution
grade
Molecular Biology
form
buffered aqueous glycerol solution
concentration
10,000 units/mL
shipped in
wet ice
storage temp.
−20°C
Application
EcoRV is a DNA restriction endonuclease used in molecular biology applications to cleave the recognition site 5′-GAT/ATC-3′, generating DNA fragments with blunt termini.
Biochem/physiol Actions
Recognition sequence: 5′-GAT/ATC-3′
Ligation and recutting results: After 2-10-fold Eco RV overdigestion of 1 μg λ DNA substrate, results in 100% cutting, >85% of fragments can be ligated, and >95% recut.
Heat inactivation: 80 °C for 20 minutes.
Ligation and recutting results: After 2-10-fold Eco RV overdigestion of 1 μg λ DNA substrate, results in 100% cutting, >85% of fragments can be ligated, and >95% recut.
Heat inactivation: 80 °C for 20 minutes.
Physical form
Solution in 20 mM Tris-HCl, pH 7.5, 1 mM EDTA, 0.1 mM PMSF, 100 mM NaCl, 10 mM 2-mercaptoethanol, 0.02% polydocanol (v/v), 0.1 mM PMSF, 50% glycerol (v/v) at 4°C
Other Notes
Supplied with 10x Restriction Endonuclease Buffer SB (B8781).
Storage Class
12 - Non Combustible Liquids
wgk
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
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I Schildkraut et al.
Gene, 27(3), 327-329 (1984-03-01)
The cleavage site for the restriction endonuclease EcoRV has been found to be 5'-GAT/ATC-3', rather than 5'- GATAT /C-3' as reported earlier by Kholmina et al. [ Dokl . Akad . Nauk . 253 (1980) 495-497].
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