R3155

Anti-Rabbit IgG, Native−Peroxidase antibody, Mouse monoclonal

clone RabT-50, purified from hybridoma cell culture

• Synonym(s): Mouse Anti-Rabbit IgG
• NACRES: NA.46
• UNSPSC Code: 12352203
• Conjugate: peroxidase conjugate
• Clone: RabT-50, monoclonal
• Application: ELISA (i), ICC, WB
• Citations: 14

Properties

• biological source: mouse
• Quality Level: 200
• conjugate: peroxidase conjugate
• antibody form: purified immunoglobulin
• antibody product type: secondary antibodies
• clone: RabT-50, monoclonal
• form: buffered aqueous solution
• species reactivity: rabbit
• should not react with: human, horse, bovine, cat, rat, chicken, pig, goat, monkey
• concentration: 2.5-5.0 mg/mL (antibody)
• technique(s): immunocytochemistry: suitable, indirect ELISA: suitable, western blot: 1:10,000-1:20,000 using 3T3 cell extracts using Anti-Actin (20-33), Catalog Number A5060, as the primary antibody, western blot: 1:10,000-1:20,000 using Hela cell extracts using Anti-Actin, N-terminal, Catalog Number A2103, as the primary antibody.
• isotype: IgG1
• shipped in: dry ice
• storage temp.: −20°C
• target post-translational modification: unmodified

Description

General description

Monoclonal Anti-Rabbit IgG, Native-Peroxidase is a purified immunoglobulin fraction of monoclonal Anti-Rabbit IgG, Native (mouse IgG1 isotype) isolated from hybridoma cell culture of the RabT-50 hybridoma, conjugated to horseradish peroxidase (HRP).

Rabbit immunoglobulins are widely used as primary antibodies in many analytical and research techniques. In immunoprecipitation assays, when target proteins are studied using rabbit primary antibody, the secondary anti-rabbit antibody used for protein detection also recognizes the heavy (50 kDa) and light (25 kDa) chains of the rabbit immunoprecipitating antibody. Consequently, this may conceal the protein of interest, as many proteins appear in the molecular weight range of the immunoglobulin heavy and light chains. The use of monoclonal anti-rabbit IgG-native-peroxidase as the secondary antibody eliminates this hindrance because the antibody is specific for non-reduced rabbit IgG and does not react with reduced rabbit IgG. Moreover, this antibody product does not react with human, monkey, bovine, horse, pig, goat, cat, rat, and chicken IgGs. Thus, this product reduces the background staining that is frequently observed with polyclonal secondary antibodies.

Immunogen

Enriched fraction of rabbit IgG light chain.

Application

Monoclonal Anti-Rabbit IgG, Native-Peroxidase antibody produced in mouse has been used in:

• immunoblotting
• immunoprecipitation
• western blotting

Biochem/physiol Actions

Monoclonal Anti-Rabbit IgG, Native-Peroxidase reacts specifically with non-reduced rabbit IgG and does not react with reduced rabbit IgG.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, and 0.05% MIT.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• pictograms: GHS07
• signalword: Warning
• hcodes: H317
• pcodes: P261 - P272 - P280 - P302 + P352 - P333 + P313 - P362 + P364
• Hazard Classifications: Skin Sens. 1
• Storage Class: 12 - Non Combustible Liquids
• wgk: WGK 3
• flash_point_f: Not applicable
• flash_point_c: Not applicable