R3380

Sigma-Aldrich

Ribonucleoside vanadyl complexes

200 mM

NACRES:
NA.52

Quality Level

concentration

200 mM

shipped in

dry ice

storage temp.

−20°C

Related Categories

General description

Ribonucleoside vanadyl complexes are stable complexes formed between ribonucleotides. They are low molecular weight inhibitors of cellular ribonucleases (RNases) that are used for the isolation of RNA from cells.

Application

Ribonucleoside vanadyl complexes are used as ribonuclease inhibitors during cell lysis and mRNA purification, to protect mRNA during cDNA production, and to protect RNA during digestion of DNA by DNaseI.
Ribonucleoside vanadyl complexes has been used:
  • as a component of lysis buffer for cell lines prior to Poly(U) pull down assay
  • to inhibit nucleases prior to immunoprecipitation (IP) of m6A-containing RNA fragment
  • as a component of hybridization buffer for human retinal slices

Biochem/physiol Actions

Ribonucleoside vanadyl complexes (RVC) elicits antimicrobial functionality by inhibiting ribosomal subunit formation and suppressing the growth of Staphylococcus aureus and Escherichia coli. It is also used as a preservative solution for tissues.

RIDADR

NONH for all modes of transport

WGK Germany

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Transcriptome-wide mapping of N6-methyladenosine by m6A-Seq
Dominissini D, et al.
Test, 560, 131-147 (2015)
SIRT1 functions as a negative regulator of eukaryotic poly (A) RNA transport
Shan P, et al.
Current Biology, 27(15), 2271-2284 (2017)
Neuroinflammatory processes, A1 astrocyte activation, and protein aggregation in the retina of Alzheimer's Disease patients, possible biomarkers for early diagnosis.
Grimaldi A, et al.
Frontiers in Neuroscience, 13, 925-925 (2019)
Effect of ribonucleoside-vanadyl complexes on enzyme-catalyzed reactions central to recombinant deoxyribonucleic acid technology.
R S Puskas et al.
Biochemistry, 21(19), 4602-4608 (1982-09-14)
Inhibition of intractable nucleases with ribonucleoside--vanadyl complexes: isolation of messenger ribonucleic acid from resting lymphocytes.
S L Berger et al.
Biochemistry, 18(23), 5143-5149 (1979-11-13)

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