R3653

Sigma-Aldrich

Anti-Radixin antibody produced in rabbit

affinity isolated antibody, buffered aqueous solution

MDL number:
NACRES:
NA.41

biological source

rabbit

Quality Level

antibody form

affinity isolated antibody

antibody product type

primary antibodies

clone

polyclonal

form

buffered aqueous solution

mol wt

antigen ~80 kDa

species reactivity

mouse, human, rat

application(s)

immunoprecipitation (IP): 10-20 μg using RIPA extract (250 μg) of rat liver
indirect immunofluorescence: 10-20 μg/mL using cultured human HeLa cells
western blot: 1-2 μg/mL using whole extract of cultured HeLa cells
western blot: 1-2 μg/mL using whole extract of cultured mouse NIH3T3 cells

conjugate

unconjugated

UniProt accession no.

shipped in

dry ice

storage temp.

−20°C

Gene Information

human ... RDX(5962)
mouse ... Rdx(19684)
rat ... Rdx(315655)

General description

RDX (radixin) belongs to the ERM (ezrin-radixin-moesin) family. It is present in several normal cells, neoplastic cells, epithelial and lymphoid cells. It is located on chromosome 11. In mouse, RDX is found in the hair cell stereocilia of inner ear.

Immunogen

synthetic peptide corresponding to amino acid residues 400-409 of human radixin with an N-terminal added tripeptide, conjugated to KLH. The corresponding sequence is identical in mouse, rat, and pig and differs by two amino acids in chicken.

Application

Anti-Radixin antibody produced in rabbit has been used in
  • immunoblotting
  • immunoprecipitation
  • immunofluorescence staining of cultured cells

Biochem/physiol Actions

Radixin is the dominant ezrin-radixin-moesin (ERM) protein in liver bile canaliculi where it is considered to have a critical role in bile conjugated-bilirubin secretion by influencing the cellular localization of the multidrug resistance protein 2 (MRP2). Resting normal human blood cells lack detectable radixin. Radixin is found in classical NK cells. Radixin has closed and open forms corresponding to the inactive and active forms as cross-linkers between actin filaments. It may be phosphorylated and it plays an important role in the activation of the Rho family members by recruiting their positive and negative regulators.
In human, knockdown of radixin (RDX) represses the development of glioblastoma cell in vitro and in vivo. It participates in cell motility, invasion and tumor progression. It acts as a membrane-cytoskeletal linker in cell surface structures that are abundant with actin. It is necessary for the arrangement of cortical cytoskeleton. RDX mutation can lead to DFNB24 hearing loss in human.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 1% bovine serum albumin and 15 mM sodium azide.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

RIDADR

NONH for all modes of transport

WGK Germany

nwg

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificate of Analysis

Certificate of Origin

Radixin knockdown by RNA interference suppresses human glioblastoma cell growth in vitro and in vivo
Qin JJ, et al.
Asian Pacific Journal of Cancer Prevention, 15(22), 9805-9812 (2014)
Radixin regulates cell migration and cell-cell adhesion through Rac1.
Valderrama F, et al.
Journal of Cell Science, 125(14), 3310-3319 (2012)
Differential expression and distribution of ezrin, radixin and moesin in human natural killer cells.
Ramoni C, et al.
European Journal of Immunology, 32(11), 3059-3065 (2002)
Structural conversion between open and closed forms of radixin: low-angle shadowing electron microscopy.
Ishikawa H, et al.
Journal of molecular biology, 310(5), 973-978 (2001)
Novel insights of the gastric gland organization revealed by chief cell specific expression of moesin
Zhu L, et al.
American Journal of Physiology: Gastrointestinal and Liver Physiology, 296(2), G185-G195 (2009)

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