R3884

Sigma-Aldrich

EclX I from Enterobacter cloacae 590

Restriction Enzyme

Enzyme Commission number:
MDL number:

grade

for molecular biology

form

buffered aqueous glycerol solution

concentration

10,000 units/mL

shipped in

wet ice

storage temp.

−20°C

Specificity

Recognition sequence: 5′-C/GGCCG-3′
Ligation and recutting results: After 2-10-fold EclX I overdigestion of 1 μg λ DNA substrate, results in 100% cutting, >95% of fragments can be ligated, and >95% recut.
Heat inactivation: 65 °C for 20 minutes.

Application

EclXI is a DNA restricition enzyme used in molecular biology applications to cleave DNA at the recognition site 5′-C/GGCCG-3′ to generate fragments with 5′-cohesive ends.

Other Notes

Supplied with 10x Restriction Enzyme Buffer SB (B8781).

Linkage

Isoschizomer: Xma III

Physical form

Solution in 20 mM Tris-HCl, pH 7.5, 0.1 mM EDTA, 100 mM NaCl, 10 mM 2-mercaptoethanol, 50% glycerol (v/v),100 μg/ml BSA, 0.05% polydocanol, 100 mg/ml hydroxy-ectione at 4°C

RIDADR

NONH for all modes of transport

WGK Germany

1

Certificate of Analysis

Certificate of Origin

EclXI, a novel isoschizomer of XmaIII from Enterobacter cloacae 590 recognizing 5'-C/GGCCG-3'.
B J Bolton et al.
Nucleic acids research, 18(2), 381-381 (1990-01-25)
Sabine Traver et al.
The Biochemical journal, 379(Pt 3), 627-632 (2004-04-29)
RGS (regulator of G-protein signalling) proteins stimulate the intrinsic GTPase activity of the a subunits of heterotrimeric G-proteins, and thereby negatively regulate G-protein-coupled receptor signalling. RGS14 has been shown previously to stimulate the GTPase activities of Ga(o) and Ga(i) subunits...
C Kessler et al.
Gene, 92(1-2), 1-248 (1990-08-16)
The properties and sources of all known class-I, class-II and class-III restriction endonucleases (ENases) and DNA modification methyltransferases (MTases) are listed and newly subclassified according to their sequence specificity. In addition, the enzymes are distinguished in a novel manner according...
Nico Mitro et al.
Methods in molecular biology (Clifton, N.J.), 952, 137-144 (2012-10-27)
The role of certain amino acids in the interactions of ligands with their cognate nuclear receptors is usually achieved by the resolution of the crystal structure of the receptor complexed with the ligand. As a complementary functional approach, site-directed mutagenesis...
Yu-Feng Huang et al.
BMC systems biology, 6 Suppl 2, S10-S10 (2013-01-11)
Current next-generation sequencing (NGS) platforms adopt two types of sequencing mechanisms: by synthesis or by ligation. The former is employed by 454 and Solexa systems, while the latter by SOLiD system. Although the pros and cons for each sequencing mechanism...

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