Reference Dye for Quantitative PCR

100 ×, solution


Quality Level




sufficient for ≥600 reactions


100 ×



storage temp.


Related Categories


Reference Dye for Quantitative PCR has been used in the preparation of mastermix for real time-quantitative polymerase chain reaction (RT-qPCR). It has also been used as a component of the reaction mixture for detection of Clostridium difficile by quantitative polymerase chain reaction (qPCR).
Sigma′s Reference Dye for qPCR is a proprietary dye for use with real-time PCR. It is used for normalization of reaction data when using SYBR Green, molecular probes, or dual-labeled probe chemistries for real-time detection. The Reference Dye is supplied as a 100× solution with a maximum excitation and emission of 586 nm and 605 nm, respectively. Instrument settings for ROX reference dye are satisfactory for the measurement of Reference Dye for qPCR.


0.3 mL in poly bottle


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Signal Word


Hazard Statements

Personal Protective Equipment

dust mask type N95 (US),Eyeshields,Gloves


NONH for all modes of transport

WGK Germany


Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Partial deletion of rng (RNase G)-enhanced homoethanol fermentation of xylose by the non-transgenic Escherichia coli RM10
Manow R, et al.
Journal of Industrial Microbiology & Biotechnology, 39(7), 977-985 (2012)
Sambrook, J. et al.
Molecular Cloning: A Laboratory Manual, 3rd (2000)
Araceli Melendez-Avalos et al.
Folia microbiologica, 65(1), 133-142 (2019-05-20)
This study aimed to analyze the proinflammatory cytokine mRNA expression in the urinary tract of BALB/c mice infected with bacterial strains with uropathogenic potential. Groups of four 6-week-old female BALB/c mice were intraurethrally inoculated with 5 × 107 colony-forming units (CFU) of...
Increased environmental sample area and recovery of Clostridium difficile spores from hospital surfaces by quantitative PCR and enrichment culture
Brown KA, et al.
Infection Control and Hospital Epidemiology : The Official Journal of the Society of Hospital Epidemiologists of America, 39(8), 917-923 (2018)
Laura K MacDougall et al.
PloS one, 13(8), e0201569-e0201569 (2018-08-31)
Contaminated surfaces serve as an important reservoir for Clostridium difficile transmission. Current strategies to detect environmental contamination of C. difficile rely heavily on culture, and often only indicate presence versus absence of spores. The goal of this study was to...
Protocol describes amplification of DNA through quantitative PCR with SYBR Green. Consistent batch-to-batch performance can be achieved with large numbers of PCR reactions.
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Our SYBR Green qPCR Protocol is a method designed to detect accurate quantification of gene expression and RT-PCR reactions
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