R4775

REDExtract-N-Amp^™ PCR ReadyMix^™

Ready-to-use 2X PCR Master Mix with Loading Dye

• Synonym(s): Direct plant PCR master mix, Direct tissue PCR master mix, PCR master mix with loading dye
• NACRES: NA.55

Properties

• Quality Level: 200
• usage: sufficient for 100 reactions; 1.2 mL sufficient for 100 amplifications; sufficient for 1000 reactions; 12 mL sufficient for 1000 amplifications; sufficient for 10000 reactions; 125 mL sufficient for 10000 amplifications
• feature: dNTPs included; hotstart
• color: red
• application(s): agriculture
• shipped in: wet ice
• storage temp.: −20°C

Description

General description

The REDExtract-N-Amp^™ PCR reaction mix is a PCR Master mix containing buffer, salts, dNTPs, and REDTaq DNA polymerase.
This PCR ReadyMix is intended for use with Sigma′s Extract-N-Amp Plant PCR kit and Extract-N-Amp Tissue PCR Kit. All Extract-N-Amp kits are sufficient for one PCR amplification per extraction. However, where additional PCR reactions are required, this supplemental PCR ReadyMix may be needed. The REDExtract-N-Amp PCR ReadyMix contains an inert tracking dye that allows for convenient direct loading of PCR products onto agarose gels for analysis.

Application

REDExtract-N-Amp^™ PCR Master Mix has been used:

• for the genotyping of fixed zebrafish embryo samples
• in polymerase chain reaction (PCR) amplification of the DNA isolated from lichen family using the REDExtract-N-Amp^™ Plant PCR Kit
• in the amplification of DNA isolated from single yeast colonies and fungal spores using REDExtract-N-Amp^™ seed PCR kit.

REDExtract-N-Amp^™ PCR Master Mix is also suitable for colony PCR.

Features and Benefits

• A Gel-ready- No loading buffer or tracking dye needs to be added. The PCR product is loaded directly into an agarose gel after amplification. The red tracer migrates at approximately the same rate as a 125bp fragment (slightly faster than bromophenol blue).
• Convenience- Hot-start feature in JumpStart^™ products allows reaction set up at room temperature, which can be beneficial when working with multiple samples. Ready-mix format reduces setup time.
• Reproducibility- Reduces the risk of contamination from multiple pipet steps and provides consistent performance across both reactions and time.
• High Specificity & Yield- JumpStart Taq DNA polymerase, an antibody inactivated hot-start enzyme, is designed to minimize non-specific amplification while increasing target yield & specificity.

Legal Information

Use of this product is covered by one or more of the following US patents and corresponding patent claims outside the US: 5,789,224, 5,618,711, 6,127,155 and claims outside the US corresponding to expired US Patent No. 5,079,352. The purchase of this product includes a limited, non-transferable immunity from suit under the foregoing patent claims for using only this amount of product for the purchaser′s own internal research. No right under any other patent claim, no right to perform any patented method, and no right to perform commercial services of any kind, including without limitation reporting the results of purchaser′s activities for a fee or other commercial consideration, is conveyed expressly, by implication, or by estoppel. This product is for research use only. Diagnostic uses under Roche patents require a separate license from Roche. Further information on purchasing licenses may be obtained by contacting the Director of Licensing, Applied Biosystems, 850 Lincoln Centre Drive, Foster City, California 94404, USA.

Extract-N-Amp is a trademark of Sigma-Aldrich Co. LLC

JumpStart is a trademark of Sigma-Aldrich Co. LLC

REDExtract-N-Amp is a trademark of Sigma-Aldrich Co. LLC

ReadyMix is a trademark of Sigma-Aldrich Co. LLC

Safety Information

• Storage Class Code: 10 - Combustible liquids
• WGK: WGK 3
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable