All Photos(1)

R5158

Sigma-Aldrich

RPMI-1640 Medium

HEPES Modification, With 25 mM HEPES and L-glutamine, liquid, sterile-filtered, suitable for cell culture

MDL number:

Quality Level

sterility

sterile-filtered

form

liquid

application(s)

cell culture | mammalian: suitable

impurities

Endotoxin, tested

pH range

>7.2

components

NaHCO3: 2 g/L
phenol red: 0.0053 g/L
L-glutamine: 0.3 g/L
glucose: 2 g/L (Dextro)
HEPES: 5.96 g/L

shipped in

ambient

storage temp.

2-8°C

Looking for similar products? Visit Product Comparison Guide

Related Categories

General description

This RPMI-1640 medium is supplemented with HEPES. HEPES is a Good′s physiological buffer that helps provide a more stable medium pH than bicarbonate buffers.

Application

RPMI 1640 Medium was developed at Roswell Park Memorial Institute in 1966 by Moore and his co-workers. A modification of McCoy′s 5A Medium, it was formulated to support lymphoblastoid cells in suspension culture, but it has since been shown to support a wide variety of cells that are anchorage dependent. Originally intended to be used with a serum supplement, RPMI 1640 has been shown to support several cell lines in the absence of serum. It has also been widely used in fusion protocols and in the growth of hybrid cells.

Linkage

Need FBS? We can assist with your serum planning. Please visit the Serum Planner for more information.

Storage Class Code

12 - Non Combustible Liquids

WGK Germany

WGK 2

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificate of Analysis

Certificate of Origin

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service