R5509

Sigma-Aldrich

Nde I from Neisseria denitrificans

Restriction Enzyme

CAS Number:
Enzyme Commission number:
MDL number:

grade

for molecular biology

form

buffered aqueous glycerol solution

concentration

10,000 units/mL

shipped in

wet ice

storage temp.

−20°C

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Specificity

Recognition sequence: 5′-CA/TATG-3′
Cutting results: a 2-10-fold Nde I overdigestion of 1 μg λ DNA substrate results in 100% cutting
Heat inactivation: Inactivated at 60 °C for 15 minutes.

Application

NdeI is a restriction endonuclease used in molecular biology methods to cleave DNA at the recognition site 5′-CA/TATG-3′, generating fragments with 5′-cohesive ends.

Other Notes

Supplied with 10x Restriction Enzyme Buffer SH (B3657).

Unit Definition

One unit is the enzyme activity that completely cleaves 1 mg λDNA in 1 hr. at 37 °C in a total volume of 25 mL of restriction endonuclease buffer SH.

Physical form

Solution in 20 mM Tris-HCl, pH 7.5, 0.1 mM EDTA, 100 mM NaCl, 1 mM dithioerythritol, 0.02% polydocanol, 0.01% gelatine, 50% glycerol (v/v) at 4 °C.

RIDADR

NONH for all modes of transport

WGK Germany

1

Certificate of Analysis

Certificate of Origin

Vega Masignani et al.
The Journal of experimental medicine, 197(6), 789-799 (2003-03-19)
Sepsis and meningitis caused by serogroup B meningococcus are devastating diseases of infants and young adults, which cannot yet be prevented by vaccination. By genome mining, we discovered GNA1870, a new surface-exposed lipoprotein of Neisseria meningitidis that induces high levels...
NdeI: a restriction endonuclease from Neisseria denitrificans which cleaves DNA at 5'-CATATG-3' sequences.
R J Watson et al.
FEBS letters, 150(1), 114-116 (1982-12-13)
Cynthia L Richard-Fogal et al.
Journal of bacteriology, 190(10), 3489-3493 (2008-03-11)
The system I cytochrome c biogenesis pathway requires CcmD, a small polypeptide of 69 residues in Escherichia coli. Here it is shown that CcmD is a component of the CcmABC ATP-binding cassette transporter complex. CcmD is not necessary for the...
C Kessler et al.
Gene, 92(1-2), 1-248 (1990-08-16)
The properties and sources of all known class-I, class-II and class-III restriction endonucleases (ENases) and DNA modification methyltransferases (MTases) are listed and newly subclassified according to their sequence specificity. In addition, the enzymes are distinguished in a novel manner according...
Saqib H Ansari et al.
Journal of pediatric hematology/oncology, 35(4), e153-e156 (2013-02-08)
β-thalassemia is characterized by impaired β-chain synthesis leading to ineffective erythropoiesis, severe anemia, and a need for blood transfusion. Presence of Xmn I polymorphism (-158 C-T nucleotide change) in γ-globin gene is associated with a higher fetal hemoglobin and a...

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