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R6148

Sigma-Aldrich

RNase A solution

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Synonym(s):
GenElute RNAse A
NACRES:
NA.52

biological source

bovine pancreas

Quality Level

form

liquid

specific activity

1500 units/mL
3000 units/mL

concentration

20 mg/mL in Tris buffer

technique(s)

DNA purification: suitable

storage temp.

room temp

Gene Information

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This Item
R5503R464211119915001
vibrant-m

R6148

RNase A solution

vibrant-m

R5503

Ribonuclease A from bovine pancreas

vibrant-m

R4642

Ribonuclease A from bovine pancreas

vibrant-m

11119915001

RNase, DNase-free

biological source

bovine pancreas

biological source

-

biological source

bovine pancreas

biological source

bovine pancreas

technique(s)

DNA purification: suitable

technique(s)

-

technique(s)

-

technique(s)

DNA purification: suitable

Gene Information

bovine ... RNASE1(282340), RNASE1(280930)

Gene Information

-

Gene Information

-

Gene Information

-

concentration

20 mg/mL in Tris buffer

concentration

-

concentration

20-40 mg/mL

concentration

-

Quality Level

200

Quality Level

300

Quality Level

200

Quality Level

100

General description

Ribonuclease A (RNase A), a protein composed of three α helices and seven β strands arranged in two "lobes” holds significance as the prototypical domain-swapped protein. It is known for its diverse oligomeric states. The RNase A family derives its name from bovine pancreatic RNase A and is specific to vertebrates. In humans, this family encompasses eight functional members, showcasing its biological relevance.
For sample preparation in GenElute kits.

Application

RNase A solution has been used:

  • in apoptosis assay of Hep-2 cells
  • for the colorimetric quantification of methylated DNA
  • for DNA and RNA extraction
  • in DNA laddering analysis
  • in cell cycle analysis
  • as a component in phosphate-buffered saline (PBS) to incubate cells for flow cytometry

Biochem/physiol Actions

Ribonuclease A or bovine pancreatic ribonuclease A (RNase A) enzyme is an endoribonuclease that displays a specific ability to cleave single-stranded RNA on the 3′ side of pyrimidine residues. This endonuclease with 124 amino acids plays a key role in gene duplication, cellular metabolism, and cancer therapy. Its overexpression is closely associated with several types of cancer. Hence, RNase A has the potential to be a promising new biomarker for the diagnosis of malignant tumors and is also used as a specific target for new drug discovery. RNase A is commonly used for the isolation of plasmid DNA.

Preparation Note

For usage requirements, please refer to the kit procedure.

Legal Information

GenElute is a trademark of Sigma-Aldrich Co. LLC

sample prep

Product No.
Description
Pricing

pictograms

Health hazard

signalword

Danger

hcodes

Hazard Classifications

Resp. Sens. 1

Storage Class

10 - Combustible liquids

wgk_germany

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable


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Homogeneous electrochemiluminescence biosensor for the detection of RNase A activity and its inhibitor
Jiancong N, et al.
Analytical Chemistry, 91, 14751-14756 (2019)
Nimesulide inhibits proliferation and induces apoptosis of pancreatic cancer cells by enhancing expression of PTEN
Chu M, et al.
Experimental and Therapeutic Medicine, 16(1), 370-376 (2018)
Determination of the presence of 5-methylcytosine in Paramecium tetraurelia
Singh A, et al.
PloS one, 13(10), e0206667-e0206667 (2018)
Bovine Pancreatic Ribonuclease A: Oxidative and Conformational Folding Studies
Scheraga HA, et al.
Methods in Enzymology, 341, 189-221 (2001)
Seasonal changes in broodstock spawning performance and egg quality in ballan wrasse (Labrus bergylta)
Grant B, et al.
Aquaculture (Amsterdam, Netherlands), 464(, 505-514 (2016)

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