R6267

RANTES human

≥97% (SDS-PAGE), recombinant, expressed in E. coli, lyophilized powder, suitable for cell culture

• Synonym(s): Regulated on Activation, Normal T-cell Expressed and Secreted
• EC Number: 200-835-2
• MDL number: MFCD00213042
• UNSPSC Code: 12352202
• NACRES: NA.32

Properties

• biological source: human
• Quality Level: 100
• recombinant: expressed in E. coli
• assay: ≥97% (SDS-PAGE)
• form: lyophilized powder
• potency: 1-5 ng/mL EC₅₀
• mol wt: 7.8-8.7 kDa
• packaging: pkg of 10 μg
• storage condition: avoid repeated freeze/thaw cycles
• technique(s): cell culture | mammalian: suitable
• impurities: endotoxin, tested
• UniProt accession no.: P13501
• storage temp.: −20°C
• Gene Information: human ... CCL5(6352)

Description

General description

Regulated on activation, normal T cell expressed and secreted (RANTES), also known as C-C motif chemokine ligand 5 (CCL5), is encoded by the gene mapped to human chromosome 17.[1] RANTES belongs to the chemokine family of proinflammatory basic polypeptides and is expressed by various hematopoietic and non-hematopoietic cell types involved in homing and migration of effector and memory T cells during acute infections.[2][3]

Biochem/physiol Actions

Regulated on activation, normal T cell expressed and secreted (RANTES) is a chemoattractant towards monocytes, dendritic cells (DCs), memory T cells (CD4⁺/CD45RO), basophils, and eosinophils. The encoded chemokine protein functions as a ligand for CCR1, CCR3, and CCR5.[1][3] RANTES inhibits the action of R5 strains of HIV-1 via blocking CCR5 receptor. A variant of RANTES gene is linked with susceptibility to HIV-1 infection and disease progression. Hence, this can be used as a potent biomarker to determine the prognosis of HIV -1 infection.[1][4]

Physical form

Lyophilized from a 0.2 μm filtered solution in 35% acetonitrile and 0.1% trifluoroacetic acid containing 0.5 mg bovine serum albumin.

Analysis Note

The biological activity is tested in culture using Baf/3 cells. Chemotaxis: EC₅₀: 1-5 ng/mL

Safety Information

• pictograms: GHS02,GHS07
• signalword: Danger
• hcodes: H225,H302 + H312 + H332,H319
• pcodes: P210 - P280 - P301 + P312 - P303 + P361 + P353 - P304 + P340 + P312 - P305 + P351 + P338
• Hazard Classifications: Acute Tox. 4 Dermal - Acute Tox. 4 Inhalation - Acute Tox. 4 Oral - Eye Irrit. 2 - Flam. Liq. 2
• Storage Class: 3 - Flammable liquids
• wgk_germany: WGK 2
• flash_point_f: 35.6 °F
• flash_point_c: 2 °C