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RPMI-1640 Medium

With L-glutamine, without sodium bicarbonate, powder, suitable for cell culture

MDL number:






cell culture | mammalian: suitable


L-glutamine: yes
phenol red: yes
NaHCO3: no
sodium pyruvate: no

shipped in


storage temp.


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General description

Roswell Park Memorial Institute medium is commonly known as RPMI. It is a form of medium used in cell culture and tissue culture. This medium contains phosphate and is formulated for use in a 5% carbon dioxide atmosphere. It is used for the growth of human lymphoid cells.


RPMI-1640 Medium is used for the following applications:
  • used in cell culture medium (i.e. Murine thymoma EL-4 cells were cultured in RPMI 1640 medium supplemented with other components)
  • used for the mycobacterial infection of splenocytes (in antibiotic-free RPMI 1640 medium)
  • used for cell isolation and culture
  • used in culture medium (as one of the component) during lymphocyte separation and culture, ELISPOT Assay, Ex Vivo proliferation assay
  • used in medium for gp39 gene expression and CD40-Immunoglobuin binding assays
  • used for the preparation of antifungal agents
RPMI 1640 Medium was developed at Roswell Park Memorial Institute in 1966 by Moore and his co-workers. A modification of McCoy′s 5A Medium, it was formulated to support lymphoblastoid cells in suspension culture, but it has since been shown to support a wide variety of cells that are anchorage dependent. Originally intended to be used with a serum supplement, RPMI 1640 has been shown to support several cell lines in the absence of serum. It has also been widely used in fusion protocols and in the growth of hybrid cells.


Formulated to contain 10.4 grams of powder per liter of medium.


Supplement with 2.0 g/L sodium bicarbonate.

Storage Class Code

11 - Combustible Solids

WGK Germany


Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificate of Analysis

Certificate of Origin

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Invasive fungal infections caused by Aspergillus (A.) and Mucorales species still represent life-threatening diseases in immunocompromised individuals, and deeper knowledge about fungal interactions with elements of innate immunity, such as complement and platelets, appears essential for optimized therapy. Previous studies...
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Scientific reports, 10(1), 18348-18348 (2020-10-29)
The infectious bursal disease (IBD) is an acute immunosuppressive viral disease that significantly affects the economics of the poultry industry. The IBD virus (IBDV) was known to infect B lymphocytes and activate macrophage and T lymphocytes, but there are limited...
M Farrington et al.
Proceedings of the National Academy of Sciences of the United States of America, 91(3), 1099-1103 (1994-02-01)
Common variable immunodeficiency (CVI) is characterized by hypogammaglobulinemia and recurrent bacterial infections due to failure of CVI B cells to differentiate in vivo into immunoglobulin-secreting plasma cells. We hypothesized that T-cell dysfunction resulting in abnormal contact-mediated B-cell activation may play...


The ELISpot Assay Enables Functional Analysis of Cellular Immunology

The enzyme-linked immunosorbent spot (ELISpot ) assay enables visualization of multiple secretory products from a single responding cell. The ELISpot provides both qualitative (type of immune protein) and quantitative (number of responding cells) information.


Powdered Media Preparation Instructions

Powdered media and salt mixtures are extremely hygroscopic and should be protected from atmospheric moisture.

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