When Sigma-Aldrich tests the activity of RNase A, a stock solution is prepared in water at 1 mg/mL. The stock solution of RNase A is also suggested can be prepared in 10 mM Tris-HCl, pH 7.5, 15 mM NaCl at a concentration of 10 mg/mL. The working concentration is suggested to be at 10 ug/ml.
R6513
Ribonuclease A from bovine pancreas
Molecular Biology, ≥70 Kunitz units/mg protein, lyophilized
Ribonuclease A from bovine pancreas
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Synonym(s):
Pancreatic Ribonuclease, RNAsea, RNase A, Ribonucleate 3′-pyrimidinooligonucleotidohydrolase
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| Pack Size | SKU | Availability | Price |
|---|---|---|---|
| 10 mg | Check Cart for Availability | $56.80 | |
| 50 mg | Check Cart for Availability | $194.00 | |
| 250 mg | Check Cart for Availability | $661.00 | |
| 500 mg | Check Cart for Availability | $1,120.00 | |
| 1 g | Check Cart for Availability | $1,910.00 |
About This Item
CAS Number:
NACRES:
NA.53
UNSPSC Code:
12352204
EC Number:
232-646-6
MDL number:
Specific activity:
≥70 Kunitz units/mg protein
grade
Molecular Biology
Quality Segment
form
lyophilized
specific activity
≥70 Kunitz units/mg protein
mol wt
13.7 kDa, ~13,700
foreign activity
Exonuclease and endonuclease, none detected, NICKase and DNase, none detected
storage temp.
−20°C
SMILES string
[nH]1cnc(c1)CC(NC(=O)CCN)C(=O)O
InChI
1S/C9H14N4O3/c10-2-1-8(14)13-7(9(15)16)3-6-4-11-5-12-6/h4-5,7H,1-3,10H2,(H,11,12)(H,13,14)(H,15,16)
InChI key
CQOVPNPJLQNMDC-UHFFFAOYSA-N
General description
RNase A is an endoribonuclease that attacks at the 3′OHphosphate of a pyrimidine nucleotide. The sequence of pG-pG-pC-pA-pG will be cleaved to give pG-pG-pCp and A-pG. The highest activity is exhibited with single stranded RNA.
RNase A, Ribonuclease A, is an endoribonuclease that cleaves the phosphodiester bonds of single strand RNA after pyrimidine nucleotides. It attacks at the 3′ phosphate end (For example pG-pG-pC-pA-pG will be cleaved to give pG-pG-pCp and A-pG). The highest activity is exhibited with single stranded RNA. RNase A is a single chain polypeptide containing 4 disulfide bridges. In contrast to RNase B, it is not a glycoprotein. Ribonucleases do not hydrolyze DNA, because the DNA lacks 2′-OH groups essential for the formation of cyclic intermediates. RNase A can also hydrolyze RNA from protein samples. RNase A can be inhibited by alkylation of His12 and His119 and activated by potassium and sodium salts. RNAse is inhibited in the presence of heavy metal ions. RNase is also inhibited competitively by DNA.
RNase A is a single chain polypeptide containing 124 amino acids, linked by four disulfide bridges. In contrast to RNase B, RNase A is not a glycoprotein. RNase A can be inhibited by alkylation of His12 or His119, which are present in the active site of the enzyme. Activators of RNase A include potassium and sodium salts.
Application
- RNase A is used to remove RNA from DNA plasmid and genomic DNA preparations and protein samples.
- RNase A is also used in RNA sequence analysis and protection assays.
- RNase A has been used as a tool for computer-aided drug design.
- RNase A supports the analysis of RNA sequences.
- RNase A hydrolyze RNA contained in protein samples.
- Purification of DNA is supported by RNase A.
Ribonuclease A from bovine pancreas has been used:
- for plasmid DNA purification
- to digest and avoid staining of double-stranded RNA during staining of nuclei[1]
- in immunoperoxidase and immunofluorescence detection
- to treat cells prior to flow cytometry
- as a component of post-hybridization buffer for washing FFPE tissue sections post in-situ hybridization
- to treat cells during the preparation of outer membrane protein (OMP) extracts
- RNase protection assays
- Removal of unspecifically bound RNA
- Analysis of RNA sequences
- Hydrolysis of RNA contained in protein samples
Features and Benefits
Our highly stable Ribonuclease A, RNase A, is suitable for removal of RNA, RNA sequencing, and DNA purification.
Analysis Note
Protein determined by E.
Other Notes
A major application for RNase A is the removal of RNA from preparations of plasmid DNA. For this application, DNase free RNase A is used at a final concentration of 10 μg/mL.
Boiling stock solutions of this RNase A product to inactivate residual DNase is not necessary and may cause precipitation of RNase and possible loss of enzymatic activity. If an RNase A solution is heated at a neutral pH, precipitation will occur. When heated at a lower pH, some precipitation may occur because of protein impurities that are present.
Boiling stock solutions of this RNase A product to inactivate residual DNase is not necessary and may cause precipitation of RNase and possible loss of enzymatic activity. If an RNase A solution is heated at a neutral pH, precipitation will occur. When heated at a lower pH, some precipitation may occur because of protein impurities that are present.
Activators of RNase A include potassium and sodium salts. RNase A can be inhibited by alkylation of His12 or His119.
1 of 1
This Item | |||
|---|---|---|---|
| specific activity ≥70 Kunitz units/mg protein | specific activity ≥70 Kunitz units/mg protein | specific activity 75-125 Kunitz units/mg protein | specific activity 85-140 Kunitz units/mg protein |
| grade Molecular Biology | grade - | grade - | grade - |
| form lyophilized | form buffered aqueous solution | form lyophilized powder | form lyophilized powder |
| mol wt 13.7 kDa, ~13,700 | mol wt ~13,700 | mol wt ~13,700 | mol wt ~13,700 |
| storage temp. −20°C | storage temp. −20°C | storage temp. −20°C | storage temp. −20°C |
| Quality Level 300 | Quality Level 200 | Quality Level 300 | Quality Level 300 |
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signalword
Danger
hcodes
pcodes
Hazard Classifications
Resp. Sens. 1
Storage Class
11 - Combustible Solids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, type N95 (US)
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Protocols
This procedure may be used for determination of Ribonuclease A (RNase A) activity.
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Instructions
Global Trade Item Number
| SKU | GTIN |
|---|---|
| R6513-1G | 04061835574797 |
| R6513-10MG | 04061835574780 |
| R6513-500MG | 04061835574810 |
| R6513-250MG | 04061835574803 |
| R6513-50MG | 04061835574827 |
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What solution should be used to dissolve RNase A? I see many options from UHP water, 0.15M NaCl, 0.01M Tris HCl, to Sodium Acetate solution. Which is preferred?
1 answer-
Helpful?
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What working concentration of Product R6513, Ribonuclease A from bovine pancreas (RNase A), do you suggest?
1 answer-
We suggest using RNase A at a concentration of 10 ug/ml.
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Do I need to boil Product R6513, Ribonuclease A from bovine pancreas (RNase A), to inactivate residual DNases?
1 answer-
Boiling of stock solutions to inactivate residual DNase is not necessary and may cause precipitation of RNase with loss of enzymatic activity.
Helpful?
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How are solutions of Product R6513, Ribonuclease A from bovine pancreas (RNase A), stored?
1 answer-
RNase A solutions can be stored, aliquoted and frozen at -20 °C.
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What is the Department of Transportation shipping information for this product?
1 answer-
Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.
Helpful?
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Why is RNase A activity not listed as micromoles per minute, and what is a Kunitz unit?
1 answer-
The Kunitz unit was created to reflect the non-linear nature of the RNAse enzymatic reaction. As the reaction progresses the activity changes due to RNA as a heterogeneous polymeric substrate; therefore, the unit of moles or micomoles per minute becomes inaccurate. As such, our enzymatic assay describes a unit that varies throughout the reaction: One unit will cause a decrease of 100% per minute in the value of E0 - Ef at pH 5.0 at 25°C. Ef is determined in the RNAse control step, while E0 reflects the current reaction rate.
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What is the shelf life of Product R6513, Ribonuclease A from bovine pancreas (RNase A)?
1 answer-
This product is stable for at least 3 years when stored properly at -20 °C.
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What is the solubility of Product R6513, Ribonuclease A from bovine pancreas (RNase A)?
1 answer-
Stock solutions of RNase A can be made in 10 mM Tris-HCl, pH 7.5, 15 mM NaCl at a concentration of 10 mg/mL.
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