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R7509

Sigma-Aldrich

RPMI-1640 Medium

Modified, with sodium bicarbonate, without L-glutamine and phenol red, liquid, sterile-filtered, suitable for cell culture

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Synonym(s):
Roswell Park Memorial Institute 1640 medium
MDL number:
NACRES:
NA.75

Quality Level

sterility

sterile-filtered

form

liquid

technique(s)

cell culture | mammalian: suitable

impurities

endotoxin, tested

components

phenol red: no
L-glutamine: no
HEPES: no
sodium pyruvate: no
NaHCO3: yes

shipped in

ambient

storage temp.

2-8°C

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This Item
R7638R7388R1145
RPMI-1640 Medium Modified, with sodium bicarbonate, without L-glutamine and phenol red, liquid, sterile-filtered, suitable for cell culture

Sigma-Aldrich

R7509

RPMI-1640 Medium

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R7638

RPMI-1640 Medium

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R7388

RPMI-1640 Medium

SAFC

SAFC

R1145

RPMI-1640 Medium

form

liquid

form

liquid

form

liquid

form

liquid

technique(s)

cell culture | mammalian: suitable

technique(s)

cell culture | mammalian: suitable

technique(s)

cell culture | mammalian: suitable

technique(s)

cell culture | mammalian: suitable

impurities

endotoxin, tested

impurities

endotoxin, tested

impurities

endotoxin, tested

impurities

endotoxin, tested

components

phenol red: no
L-glutamine: no
HEPES: no
sodium pyruvate: no
NaHCO3: yes

components

sodium pyruvate: no
HEPES: 20 mM
L-glutamine: no
NaHCO3: yes
phenol red: yes

components

HEPES: 20 mM
NaHCO3: no
sodium pyruvate: no
phenol red: yes
L-glutamine: yes

components

NaHCO3: no
phenol red: yes
sodium pyruvate: no
HEPES: no
L-glutamine: no

shipped in

ambient

shipped in

ambient

shipped in

ambient

shipped in

ambient

General description

RPMI 1640 Medium was developed at Roswell Park Memorial Institute in 1966 by Moore and his co-workers. A modification of McCoy′s 5A Medium, it was formulated to support lymphoblastoid cells in suspension culture, but it has since been shown to support a wide variety of cells that are anchorage-dependent. Originally intended to be used with a serum supplement, RPMI 1640 has been shown to support several cell lines in the absence of serum. It has also been widely used in fusion protocols and in the growth of hybrid cells. This medium is suitable for culturing human normal and neoplastic leukocytes.
RPMI-1640 Medium is suitable for culturing human normal and neoplastic leukocytes. 

Application

RPMI-1640 Medium has been used:
  • to culture Mantle cell lymphoma cells
  • to culture mouse podocytes
  • in the extraction of whole bone marrow from rat Femur and tibia bones

Other Notes

Phenol red has been shown to interfere with the growth of some cells at clonal densities.
Use this medium when working with stem cells or when growing cells at low densities.
Also recommended for in vitro diagnostics use.

Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable


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