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RPMI-1640 Medium

Modified, with sodium bicarbonate, without L-glutamine and phenol red, liquid, sterile-filtered, suitable for cell culture

MDL number:

Quality Level






cell culture | mammalian: suitable


endotoxin, tested


phenol red: no
L-glutamine: no
sodium pyruvate: no
NaHCO3: yes

shipped in


storage temp.


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RPMI 1640 Medium was developed at Roswell Park Memorial Institute in 1966 by Moore and his co-workers. A modification of McCoy′s 5A Medium, it was formulated to support lymphoblastoid cells in suspension culture, but it has since been shown to support a wide variety of cells that are anchorage dependent. Originally intended to be used with a serum supplement, RPMI 1640 has been shown to support several cell lines in the absence of serum. It has also been widely used in fusion protocols and in the growth of hybrid cells.

Other Notes

Phenol red has been shown to interfere with the growth of some cells at clonal densities.
Use this medium when working with stem cells or when growing cells at low densities.
Also recommended for in vitro diagnostics use.

Storage Class Code

12 - Non Combustible Liquids

WGK Germany


Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificate of Analysis

Certificate of Origin

Ingvild S M Gabrielsen et al.
PloS one, 14(7), e0218858-e0218858 (2019-07-02)
Antigen presenting cells (APCs) in the thymus play an essential role in the establishment of central tolerance, i.e. the generation of a repertoire of functional and self-tolerant T cells to prevent autoimmunity. In this study, we have compared the transcriptomes...
Hubert Stangl et al.
Scientific reports, 10(1), 17071-17071 (2020-10-15)
In experimental arthritis, glucocorticoid secretion is inadequate relative to inflammation. We hypothesized that IL-1 is a key factor for inadequate glucocorticoid secretion in arthritic rats. Collagen type II-induced arthritis (CIA) in DA rats was the model to study effects of...
Ida Marie Rundgren et al.
Journal of immunological methods, 461, 53-62 (2018-06-16)
Monocytes are important for innate immunity and include the classical (CD14brightCD16negative), intermediate (CD14brightCD16dim) and non-classical (CD14dimCD16bright) monocyte subsets. The quantification of these functionally different subsets in peripheral blood may become useful for diagnosis and follow-up in human diseases. The aim...
Alyson A Fiorillo et al.
Frontiers in immunology, 11, 151-151 (2020-03-11)
MicroRNAs (miRNAs) are small non-coding RNA molecules that regulate important intracellular biological processes. In myasthenia gravis (MG), a disease-specific pattern of elevated circulating miRNAs has been found, and proposed as potential biomarkers. These elevated miRNAs include miR-150-5p, miR-21-5p, and miR-30e-5p...
Jangsoon Lee et al.
Biomedicines, 9(7) (2021-07-03)
Human epidermal growth factor receptor (EGFR) 2 (HER2) is overexpressed/amplified in about 25% of all breast cancers, and EGFR is overexpressed in up to 76% and amplified in up to 24% of triple-negative breast cancers (TNBC). Here, we aimed to...

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