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RPMI-1640 Medium

AutoMod, without L-glutamine and sodium bicarbonate, powder, suitable for cell culture

MDL number:

Quality Level




cell culture | mammalian: suitable


L-glutamine: no
NaHCO3: no
sodium pyruvate: no
phenol red: yes

shipped in


storage temp.


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RPMI 1640 Medium was developed at Roswell Park Memorial Institute in 1966 by Moore and his co-workers. A modification of McCoy′s 5A Medium, it was formulated to support lymphoblastoid cells in suspension culture, but it has since been shown to support a wide variety of cells that are anchorage dependent. Originally intended to be used with a serum supplement, RPMI 1640 has been shown to support several cell lines in the absence of serum. It has also been widely used in fusion protocols and in the growth of hybrid cells.

Other Notes

Modified for autoclaving
Without L-glutamine and sodium bicarbonate.


Formulated to contain 10.3 grams of powder per liter of medium.


Supplement with 0.3 L-glutamine, 2.0 g/L sodium bicarbonate.

Legal Information

Auto-Mod is a trademark of Sigma-Aldrich Co. LLC

Storage Class Code

11 - Combustible Solids



Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificate of Analysis

Certificate of Origin

Zhonghua Liu et al.
mBio, 12(3) (2021-05-20)
Inhibition of fungal growth by Congo red (CR) has been putatively associated with specific binding to β-1,3-glucans, which blocks cell wall polysaccharide synthesis. In this study, we searched for transcription factors (TFs) that regulate the response to CR and interrogated
Leenah Alaalm et al.
Frontiers in cellular and infection microbiology, 11, 637836-637836 (2021-09-14)
The highly conserved, ubiquitous molecular chaperone Hsp90 is a key regulator of cellular proteostasis and environmental stress responses. In human pathogenic fungi, which kill more than 1.6 million patients each year worldwide, Hsp90 governs cellular morphogenesis, drug resistance, and virulence.
Karla Lucía F Alvarez et al.
BMC veterinary research, 16(1), 230-230 (2020-07-08)
In the poultry industry, quantitative analysis of chicken T cell proliferation is important in many biological applications such as drug screening, vaccine production, and cytotoxicity assessment. Several assays have been established to evaluate this immunological response in chicken cells. However
Michael D Lewis et al.
The American journal of tropical medicine and hygiene, 81(6), 1041-1049 (2009-12-10)
Trypanosoma cruzi, the agent of Chagas disease, can be subdivided into six discrete typing units (DTUs), TcI, TcIIa, TcIIb, TcIIc, TcIId or TcIIe, each having distinct epidemiologically important features. Dozens of genetic markers are available to determine the DTU to
Sven D Willger et al.
PLoS pathogens, 4(11), e1000200-e1000200 (2008-11-08)
At the site of microbial infections, the significant influx of immune effector cells and the necrosis of tissue by the invading pathogen generate hypoxic microenvironments in which both the pathogen and host cells must survive. Currently, whether hypoxia adaptation is


Powdered Media Preparation Instructions

Powdered media and salt mixtures are extremely hygroscopic and should be protected from atmospheric moisture.

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