The amino acid sequence for the two enzymes is the same. The difference between them is that RNase B is a glycoprotein and contains 6 mannose residues and 2 residues of N-acetylglucosamine.
R7884
Ribonuclease B from bovine pancreas
BioReagent, ≥50 Kunitz units/mg protein, ≥80% (SDS-PAGE)
Synonym(s):
RNase B
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R7884-500UG
$58.10
R7884-100MG
$792.00
$58.10
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biological source
bovine pancreas
Quality Level
product line
BioReagent
assay
≥80% (SDS-PAGE)
form
powder
specific activity
≥50 Kunitz units/mg protein
concentration
≥60%
technique(s)
cell based assay: suitable
suitability
suitable for molecular biology
application(s)
cell analysis
foreign activity
protease ≤0.001 units/mg solid
storage temp.
−20°C
SMILES string
[nH]1cncc1CC(NC(=O)CCN)C(=O)O
InChI
1S/C9H14N4O3/c10-2-1-8(14)13-7(9(15)16)3-6-4-11-5-12-6/h4-5,7H,1-3,10H2,(H,11,12)(H,13,14)(H,15,16)
InChI key
CQOVPNPJLQNMDC-UHFFFAOYSA-N
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1 of 4
This Item | R5500 | R5000 | R5503 |
|---|---|---|---|
| assay ≥80% (SDS-PAGE) | assay ≥90% (SDS-PAGE) | assay - | assay - |
| specific activity ≥50 Kunitz units/mg protein | specific activity 75-125 Kunitz units/mg protein | specific activity >= 60 Kunitz units/mg protein | specific activity 50-100 Kunitz units/mg protein |
| technique(s) cell based assay: suitable | technique(s) cell based assay: suitable | technique(s) cell based assay: suitable | technique(s) cell based assay: suitable |
| biological source bovine pancreas | biological source bovine pancreas | biological source bovine pancreas | biological source bovine pancreas |
| form powder | form lyophilized powder | form solid | form lyophilized powder |
| application(s) cell analysis | application(s) diagnostic assay manufacturing | application(s) diagnostic assay manufacturing | application(s) diagnostic assay manufacturing |
Application
Ribonuclease B from bovine pancreas is used in the digestion of RNA during cell cycle platform analysis.[1]
Biochem/physiol Actions
Native RNase BS generated by subtilisin digestion of native RNase B comprising of amino acid residues 21-124 of RNase B, is sensitive to PNGase F digestion.[2] Intramolecular N-glycans of bovine pancreatic RNase B function like chaperone. RNase B is found to be much faster than RNase A, while RNase A is liable to aggregate during regeneration. The stimulatory effect of Asn-oligosaccharide (which corresponds to the most predominant sugar chain of RNase B) reveals that the N-glycans of RNase B facilitates the transformation of bulky intermediates into folded, compact species.[3]
Packaging
Package size based on protein content
Preparation Note
Purified by affinity chromatography
related product
Product No.
Description
Pricing
signalword
Danger
hcodes
pcodes
Hazard Classifications
Resp. Sens. 1
Storage Class
11 - Combustible Solids
wgk_germany
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, type N95 (US)
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Véronique Blanchard et al.
Biochemistry, 47(11), 3435-3446 (2008-02-26)
In glycoanalysis protocols, N-glycans from glycoproteins are most frequently released with peptide- N (4)-( N-acetyl-beta-glucosaminyl)asparagine amidase F (PNGase F). As the enzyme is an amidase, it cleaves the NH-CO linkage between the Asn side chain and the Asn-bound GlcNAc residue.
Audra A Hargett et al.
Molecules (Basel, Switzerland), 26(14) (2021-07-25)
Protein glycosylation is important in many organisms for proper protein folding, signaling, cell adhesion, protein-protein interactions, and immune responses. Thus, effectively determining the extent of glycosylation in glycoprotein therapeutics is crucial. Up to now, characterizing protein glycosylation has been carried
Yang Xu et al.
iScience, 25(8), 104753-104753 (2022-08-10)
N-Acetylglucosamine (GlcNAc) is an essential monosaccharide required in almost all organisms. Fluorescent labeling of the peptidoglycan (PG) on N-acetylglucosamine has been poorly explored. Here, we report on the labeling of the PG with a bioorthogonal handle on the GlcNAc. We
H Yamaguchi et al.
Journal of biochemistry, 120(3), 474-477 (1996-09-01)
This paper describes a chaperone-like function of the intramolecular N-glycans of bovine pancreatic RNase B. We studied air-oxidative regeneration from reductively denatured species of RNase B and its nonglycosylated form, RNase A. RNase B was reactivated much faster than RNase
Rafał Kolenda et al.
Frontiers in microbiology, 9, 1905-1905 (2018-09-07)
Bacterial host tropism is a primary determinant of the range of host organisms they can infect. Salmonella serotypes are differentiated into host-restricted and host-adapted specialists, and host-unrestricted generalists. In order to elucidate the underlying molecular mechanisms of host specificity in
Articles
PNGase Fast denaturing buffer and enzyme provide results similar to a conventional 20-hour protocol, reducing workflow time to about 1 hour.
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What is the difference between RNase B, Ribonuclease B from bovine pancreas, Product R7884, and RNase A?
1 answer-
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What is the extinction coefficient of Ribonuclease B from bovine pancreas (RNase B), Product R7884?
1 answer-
The E1% for this enzyme is 8.0 when measured at 280 nm.
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How should Ribonuclease B from bovine pancreas, Product R7884, be reconstituted?
1 answer-
This product may be reconstituted directly in water at a concentration of 1 mg/mL.
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What is the Department of Transportation shipping information for this product?
1 answer-
Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.
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