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R8005

SAFC

RPMI-1640 Medium

Hybri-Max™, Modified, with L-glutamine, 4500 mg/L glucose and 15mM HEPES, without sodium bicarbonate, powder, suitable for hybridoma

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MDL number:
MFCD00217820
NACRES:
NA.75

Quality Level

500

form

powder

technique(s)

cell culture | hybridoma: suitable

impurities

endotoxin

components

phenol red: 0.0053 g/L
L-glutamine: 0.3 g/L
HEPES: 3.5745 g/L
NaHCO3: no
glucose: 4.5 g/L (Dextro)

shipped in

ambient

storage temp.

2-8°C

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SAFC

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R8005

RPMI-1640 Medium

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R7513

RPMI-1640 Medium

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R1780

RPMI-1640 Medium

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R1145

RPMI-1640 Medium

technique(s)

cell culture | hybridoma: suitable

technique(s)

cell culture | mammalian: suitable

technique(s)

cell culture | mammalian: suitable

technique(s)

cell culture | mammalian: suitable

impurities

endotoxin

impurities

endotoxin, tested

impurities

endotoxin, tested

impurities

endotoxin, tested

components

phenol red: 0.0053 g/L
L-glutamine: 0.3 g/L
HEPES: 3.5745 g/L
NaHCO3: no
glucose: 4.5 g/L (Dextro)

components

HEPES: no
sodium pyruvate: no
phenol red: yes
L-glutamine: no
NaHCO3: yes

components

NaHCO3: 2 g/L
phenol red: no
glucose: 2 g/L (Dextro)
L-glutamine: 0.3 g/L

components

NaHCO3: no
phenol red: yes
sodium pyruvate: no
HEPES: no
L-glutamine: no

shipped in

ambient

shipped in

ambient

shipped in

ambient

shipped in

ambient

storage temp.

2-8°C

storage temp.

2-8°C

storage temp.

-

storage temp.

2-8°C

General description

RPMI 1640 Medium was developed at Roswell Park Memorial Institute in 1966 by Moore and his co-workers. A modification of McCoy′s 5A Medium, it was formulated to support lymphoblastoid cells in suspension culture, but it has since been shown to support a wide variety of cells that are anchorage-dependent. Originally intended to be used with a serum supplement, RPMI 1640 has been shown to support several cell lines in the absence of serum. It has also been widely used in fusion protocols and in the growth of hybrid cells. This medium is suitable for culturing human normal and neoplastic leukocytes.

Quantity

Formulated to contain 16.4 grams of powder per liter of medium.

Reconstitution

Supplement with 2.0 g/L sodium bicarbonate.

Legal Information

Hybri-Max is a trademark of Sigma-Aldrich Co. LLC

also commonly purchased with this product

Product No.
Description
Pricing

supplement

Product No.
Description
Pricing

Storage Class Code

11 - Combustible Solids

WGK

WGK 2

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificates of Analysis (COA)

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T1503
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25G
Pack Size/Quantity

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1000309185

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RPMI 1640 Complete Medium, with 2mM L-Glut and 10% FBS RPMI 1640 Complete Medium is supplemented with 2mM L-Glutamine and 10% U.S. origin FBS. It is a convenient sterile solution for the culture of a wide variety of cells that grow in suspension or as anchorage dependent, and can be used in fusion protocols and in the growth of hybrid cells.

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RPMI 1640 Complete Medium, with 2mM L-Glut and 10% FBS

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RPMI-1640 Medium

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