Documents

R9376

Sigma-Aldrich

AMV-Reverse Transcriptase from avian myeloblastosis virus

Synonym(s):
RNA-directed DNA polymerase, Revertase
CAS Number:
Enzyme Commission number:
MDL number:

form

solution

concentration

10,000-100,000 units/mL

shipped in

dry ice

storage temp.

−70°C

Application

AMV reverse transcriptase synthesizes DNA complementary (cDNA) to RNA templates. A DNA primer complementary to the RNA template and a divalent cation, either Mg or Mn, are required for initiation of transcription. This enzyme is commonly used to make cDNAs from mRNA for eventual cloning or for use as probes.

Unit Definition

One unit incorporates 1 nmol of dTTP into TCA precipitable material in 10 min at 37 °C using polyadenylic acid as template and oligo(dT)12-18 as a primer.

Legal Information

Purchase of this product is accompanied by a limited license for use in the Polymerase Chain Reaction (PCR) process for research purposes only and in conjunction with a thermal cycler whose use in the automated performance of the PCR process is covered by an up-front license fee, either by payment to Applied Biosystems or as purchased, i.e., and authorized thermal cycler.

WGK Germany

1

Ausubel, F., et al.
Short Protocols in Molecular Biology, 14-14 (1995)
Xiu-Juan Jiao et al.
Zhonghua shi yan he lin chuang bing du xue za zhi = Zhonghua shiyan he linchuang bingduxue zazhi = Chinese journal of experimental and clinical virology, 26(6), 453-455 (2013-05-01)
To characterize genotypic resistance within HBV RT region in chronic hepatitis B (CHB) patients with nucleos(t)ide analogue (NA) treatment. Serum samples of 229 CHB patients with NA treatment were obtained. Full-length HBV RT sequences were amplified, sequenced and analyzed, on...
TERT hypermethylation: biomarker in paediatric brain tumours.
Scott J Diede
The Lancet. Oncology, 14(6), 447-448 (2013-04-20)
Jiansen Jiang et al.
Nature, 496(7444), 187-192 (2013-04-05)
Telomerase adds telomeric repeats to chromosome ends using an internal RNA template and a specialized telomerase reverse transcriptase (TERT), thereby maintaining genome integrity. Little is known about the physical relationships among protein and RNA subunits within a biologically functional holoenzyme....
Oumar Samassekou et al.
Journal of hematology & oncology, 6, 26-26 (2013-04-04)
The predominant mechanism by which human tumors maintain telomere length is via telomerase. In ~10% of tumor samples, however, telomere length is conserved, despite no detectable telomerase activity, in part through activation of the alternative lengthening of telomeres (ALT) pathway....

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