R9894

Sigma-Aldrich

Ras human

Wild type, recombinant, expressed in E. coli, buffered aqueous glycerol solution

Synonym(s):
H-Ras, rH-RAS, WT
MDL number:
NACRES:
NA.32
Pricing and availability is not currently available.

Quality Level

recombinant

expressed in E. coli

assay

≥80% (SDS-PAGE)

form

buffered aqueous glycerol solution

mol wt

28 kDa

shipped in

dry ice

storage temp.

−70°C

Gene Information

human ... RASGEF1B(153020)

General description

Ras proto-oncogene is a guanine-nucleotide binding protein that couples tyrosine kinase receptor signals to mitogen activated protein (MAP) kinase cascade. It has an important role in cell growth. The Ras gene is localized on human chromosome 11p15.5. Ras is a substrate for farnesyltransferase (FTase) and when farnesylated, it is anchored to the membrane.

Application

Human Ras has been used as a negative control in tubulin overlay assay.

Biochem/physiol Actions

Ras proteins, p21, are guanyl nucleotide binding proteins that play an important role in cellular signal transduction. Ras proteins are regulatory proteins that bind GTP in their active state. A Ras intrinsic GTPase activity hydrolyzes guanosine-5′-triphosphate (GTP) to guanosine diphosphate (GDP) thus inactivating Ras, while activation of Ras requires exchange of the bound GDP with GTP. Inhibition of Ras activity by stimulation of its GTPase activity is enhanced by GTPase activating proteins. Membrane localization of Ras is crucial for its cellular activity. Inhibition of Ras farnesylation is a target for anti-cancer drug development, since abnormally active Ras is present in more than 50% of human cancers.

Packaging

Package size based on protein content

Physical form

Solution in 50% glycerol containing 50 mM Tris (pH 8.0), 5 mM MgCl2, 45 mM NaCl, 1 mM DTT

RIDADR

NONH for all modes of transport

WGK Germany

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificate of Analysis
Certificate of Origin
Real-time in vitro measurement of intrinsic and Ras GAP-mediated GTP hydrolysi
Shutes A, et al.
Test (2006)
Human Mucin Gene MUC5B, the 10.7-kb Large Central Exon Encodes Various Alternate Subdomains Resulting in a Super-repeat
Jean-Luc D
The Journal of Biological Chemistry (1997)
Real-time in vitro measurement of intrinsic and Ras GAP-mediated GTP hydrolysi
Shutes A, et al.
Methods in Enzymology (2006)
A role for the vesicle-associated tubulin binding protein ARL6 (BBS3) in flagellum extension in Trypanosoma brucei
Helen P.Price
Biochim. Biophys. Acta Gen. Subj. (2012)
Inactivation of Ras GTPase-activating proteins promotes unrestrained activity of wild-type Ras in human liver cancer
Calvisi DF, et al.
Journal of Hepatology, 54(2), 311-311 (2011)
Articles
We present an article about how proliferating cells require the biosynthesis of structural components for biomass production and for genomic replication.
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