All Photos(2)

RAB0001

Sigma-Aldrich

Human IgG ELISA Kit

for serum, plasma

NACRES:
NA.32

species reactivity

human

application(s)

ELISA: suitable
capture ELISA: suitable

shipped in

wet ice

storage temp.

−20°C

General description

This ELISA (enzyme-linked immunosorbent assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of a target protein in biological samples such as serum, plasma, cell culture supernatants, urine and/or cell and tissue lysates. This assay employs a specific capture antibody coated on a 96 well plate.
Immunoglobulin G (IgG) belongs to immunoglobulin family, and consists of gamma (γ) heavy chain in the constant (C) region. IgG is a widely expressed serum antibody. Monomeric structure of IgG constitutes two identical heavy chains and two identical light chains with molecular weights of 50kDa and 25kDa respectively. The primary structure of this antibody also contains disulfide bonds linking the two heavy chains, linking the heavy and light chains, and are present inside the chains. IgG is further subdivided into four classes namely, IgG1, IgG2, IgG3, and IgG4 with different heavy chains, named γ1, γ2, γ3, andγ4, respectively. IgG is produced and released from CD5+ B cells, the predominant lymphocytes in the neonatal cell repertoire. Maternal IgG is the only antibody transported across the placenta to the fetus. Maternal IgG passively immunizes the infants.

Immunogen

Recombinant Human IGG

Application

Human IgG ELISA Kit has been used to determine the total IgG antibody levels in serum samples of new borns. It has also been used to validate the micro-bead injection spectroscopy (μ-BIS)- lab-on-valve (LOV) method for the determination of serum immunoglobulin G (IgG).

Other Notes

A sample Certificate of Analysis is available for this product.
Please type the word sample in the text box provided for lot number.

Kit Components Also Available Separately

Product No.
Description
SDS

  • RABWASH420X Wash Buffer (Item B)

  • RABELADEELISA 5X Assay/Sample Diluent Buffer E (Item E2)

  • RABTMB3ELISA Colorimetric TMB Reagent (HRP Substrate, Item H)

  • RABSTOP3ELISA Stop Solution (Item I)

Pictograms

Corrosion

Signal Word

Warning

Hazard Statements

Hazard Classifications

Met. Corr. 1

Storage Class Code

8B - Non-combustible, corrosive hazardous materials

Certificate of Analysis

Certificate of Origin

Phoebe E Lewis et al.
Vaccines, 8(2) (2020-06-11)
Studies have shown that blockade of CTLA-4 promoted the expansion of germinal center B-cells in viral infection or immunization with model antigens. Few studies have evaluated the immunological consequences of CTLA-4 blockade during immunization against relevant vaccine candidates. Here, we...
Narmeen Mallah et al.
Frontiers in pharmacology, 11, 860-860 (2020-07-14)
Despite the wide benefits of aspirin and its cost-effectiveness, aspirin prescriptions have been reduced due to idiosyncratic responses in susceptible individuals. Low-dose aspirin and single-nucleotide polymorphisms (SNPs) are independently associated with increased risk of gastrointestinal hemorrhage; however, to-date, no studies...
Franck P Dupuy et al.
mBio, 10(6) (2019-12-19)
Measuring Envelope (Env)-specific antibody (Ab)-dependent cellular cytotoxicity (ADCC)-competent Abs in HIV+ plasma is challenging because Env displays distinctive epitopes when present in a native closed trimeric conformation on infected cells or in a CD4-bound conformation on uninfected bystander cells. We...
The repertoire of maternal anti-viral antibodies in human newborns
Nature Medicine, 25, 591-591 (2019)
Katharina Kappler et al.
Frontiers in microbiology, 11, 1553-1553 (2020-08-09)
Inflammatory bowel disease is associated with intestinal dysbiosis and with elevated antibody production toward microbial epitopes. The underlying processes linking the gut microbiota with inflammation are still unclear. Considering the constant induction of antibodies by gut microbial glycans, the aim...

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