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RAB0002

Sigma-Aldrich

Mouse ACE  ELISA Kit

for serum, plasma and cell culture supernatant

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NACRES:
NA.32

species reactivity

mouse

packaging

kit of 96 wells (12 strips x 8 wells)

technique(s)

ELISA: suitable
capture ELISA: suitable

input

sample type cell culture supernatant(s)
sample type serum
sample type plasma

assay range

inter-assay cv: <12%
intra-assay cv: <10%
sensitivity: 0.12 ng/mL
standard curve range: 0.123-30 ng/mL

detection method

colorimetric

shipped in

wet ice

storage temp.

−20°C

Gene Information

mouse ... Ace(11421)

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Gene Information

mouse ... Ace(11421)

Gene Information

mouse ... Egf(13645)

Gene Information

mouse ... Ager(11596)

Gene Information

mouse ... Il3(16187)

input

sample type cell culture supernatant(s)
sample type serum
sample type plasma

input

sample type cell culture supernatant(s)
sample type plasma
sample type serum

input

sample type serum
sample type plasma
sample type cell culture supernatant(s)

input

sample type serum
sample type plasma
sample type cell culture supernatant(s)

assay range

inter-assay cv: <12%
intra-assay cv: <10%
sensitivity: 0.12 ng/mL
standard curve range: 0.123-30 ng/mL

assay range

inter-assay cv: <12%
intra-assay cv: <10%
sensitivity: 3 pg/mL
standard curve range: 3.28-800 pg/mL

assay range

inter-assay cv: <12%
intra-assay cv: <10%
sensitivity: 15 pg/mL
standard curve range: 13.72-10000 pg/mL

assay range

inter-assay cv: <12%
intra-assay cv: <10%
sensitivity: 0.2 pg/mL
standard curve range: 0.16-40 pg/mL

technique(s)

ELISA: suitable, capture ELISA: suitable

technique(s)

ELISA: suitable, capture ELISA: suitable

technique(s)

ELISA: suitable, capture ELISA: suitable

technique(s)

ELISA: suitable, capture ELISA: suitable

detection method

colorimetric

detection method

colorimetric

detection method

colorimetric

detection method

colorimetric

General description

The Mouse ACE (Angiotensin-Converting Enzyme) ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of mouse ACE in serum, plasma, cell culture supernatants and urine.

Immunogen

Recombinant Mouse ACE

Application

For research use only. Not for use in diagnostic procedures.
Please refer to the attached General ELISA KIT Procedure (sandwich, competitive & Indirect ELISA)
Mouse ACE ELISA Kit has been used to quantify total serum concentration of angiotensin converting enzyme (ACE) in spontaneously hypertensive rats.

Biochem/physiol Actions

Angiotensin I converting enzyme (ACE) catalyzes the formation of angiotensin II from angiotensin I. ACE is a key aminopeptidase of the renin-angiotensin system. It is a membrane-bound enzyme which is expressed in atherosclerotic lesions.

Other Notes

A sample Certificate of Analysis is available for this product.
Please type the word sample in the text box provided for lot number.

Kit Components Also Available Separately

Product No.
Description
SDS

  • RABELADAELISA 1X Assay/Sample Diluent Buffer A (Item D1)SDS

  • RABELADBELISA 5X Assay/Sample Diluent Buffer B (Item E1)SDS

  • RABSTOP3ELISA Stop Solution (Item I)SDS

  • RABTMB3ELISA Colorimetric TMB Reagent (HRP Substrate, Item H)SDS

  • RABWASH420X Wash Buffer (Item B)SDS

pictograms

Corrosion

signalword

Warning

hcodes

Hazard Classifications

Met. Corr. 1

Storage Class

8A - Combustible, corrosive hazardous materials

wgk_germany

WGK 3


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Nandadeva Lokugamage et al.
Vaccines, 8(1) (2020-02-27)
Chagas cardiomyopathy is caused by Trypanosoma cruzi (Tc). We identified two candidate antigens (TcG2 and TcG4) that elicit antibodies and T cell responses in naturally infected diverse hosts. In this study, we cloned TcG2 and TcG4 in a nanovector and
Angiotensin-Converting Enzyme in Smooth Muscle Cells Promotes Atherosclerosis
Xiaoofeng Chen
Arteriosclerosis, Thrombosis, and Vascular Biology (2016)
Local corticosterone production and angiotensin-I converting enzyme shedding in a mouse model of intestinal inflammation
Hanne Salmenkari
World Journal of Gastroenterology (2015)
Leah K Cuddy et al.
Science translational medicine, 12(563) (2020-10-02)
Recent genome-wide association studies identified the angiotensin-converting enzyme gene (ACE) as an Alzheimer's disease (AD) risk locus. However, the pathogenic mechanism by which ACE causes AD is unknown. Using whole-genome sequencing, we identified rare ACE coding variants in AD families
A study on the effect of the Dendropanax mobifera extract on anti-hypertensive
Jo YB and Lee JH
Journal of the Korea Academia-Industrial cooperation Society, 17, 708-715 (2016)

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