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RAB0017

Sigma-Aldrich

Human ANGPTL4 ELISA Kit

for serum, plasma and cell culture supernatants

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NACRES:
NA.32

species reactivity

human

packaging

kit of 96 wells (12 strips x 8 wells)

technique(s)

ELISA: suitable
capture ELISA: suitable

input

sample type cell culture supernatant(s)
sample type serum
sample type plasma

assay range

inter-assay cv: <12%
intra-assay cv: <10%
sensitivity: 20 pg/mL
standard curve range: 27.4-20000 pg/mL

detection method

colorimetric

shipped in

wet ice

storage temp.

−20°C

Gene Information

human ... ANGPTL4(51378)

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RAB0531RAB0606RAB0756
packaging

kit of 96 wells (12 strips x 8 wells)

packaging

kit of 96 wells (12 strips x 8 wells)

packaging

kit of 96 wells (12 strips x 8 wells)

packaging

kit of 96 wells (12 strips x 8 wells)

technique(s)

ELISA: suitable, capture ELISA: suitable

technique(s)

ELISA: suitable

technique(s)

ELISA: suitable

technique(s)

ELISA: suitable

input

sample type cell culture supernatant(s)
sample type serum
sample type plasma

input

sample type urine
sample type serum
sample type cell culture supernatant(s)
sample type plasma

input

sample type plasma
sample type cell culture supernatant(s)
sample type serum

input

sample type cell culture supernatant(s)
sample type plasma
sample type serum

assay range

inter-assay cv: <12%
intra-assay cv: <10%
sensitivity: 20 pg/mL
standard curve range: 27.4-20000 pg/mL

assay range

inter-assay cv: <10%
intra-assay cv: <12%
sensitivity: 0.25 ng/mL

assay range

inter-assay cv: <12%
intra-assay cv: <10%
sensitivity: 8.2 pg/mL
standard curve range: 8.19-2000 pg/mL

assay range

inter-assay cv: <12%
intra-assay cv: <10%
sensitivity: 40 pg/mL
standard curve range: 40.96-10000 pg/mL

detection method

colorimetric

detection method

colorimetric

detection method

colorimetric

detection method

colorimetric

General description

The Human ANGPTL4 (Angiopoietin-like 4) ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of human ANGPTL4 in serum, plasma and cell culture supernatants.

Immunogen

Recombinant Human Angiopoietin-like 4

Application

For research use only. Not for use in diagnostic procedures.
Human angiopoietin-like protein 4 (ANGPTL4) ELISA kit has been used for the quantitative measurement of ANGPTL4 in serum and urinary samples.

Biochem/physiol Actions

Angiopoietin like 4 (ANGPTL4) possesses a characteristic large fibrinogen/angiopoietin like domain. It inhibits lipoprotein lipase and thus increases plasma triglyceride levels. The protein regulates the uptake of free fatty acids in fasting and fed conditions. ANGPTL4 is also involved in homeostasis, insulin sensitivity and apoptosis of vascular endothelial cells. It has been reported to have a role in atherosclerosis and may modulate inflammatory responses.

Other Notes

A sample Certificate of Analysis is available for this product.
Please type the word sample in the text box provided for lot number.

Kit Components Also Available Separately

Product No.
Description
SDS

  • RABELADAELISA 1X Assay/Sample Diluent Buffer A (Item D1)SDS

  • RABELADBELISA 5X Assay/Sample Diluent Buffer B (Item E1)SDS

  • RABSTOP3ELISA Stop Solution (Item I)SDS

  • RABTMB3ELISA Colorimetric TMB Reagent (HRP Substrate, Item H)SDS

  • RABWASH420X Wash Buffer (Item B)SDS

Pictograms

Corrosion

Signal Word

Warning

Hazard Statements

Precautionary Statements

Hazard Classifications

Met. Corr. 1

Storage Class Code

8A - Combustible, corrosive hazardous materials

WGK

WGK 3


Certificates of Analysis (COA)

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Viktoria Gusarova et al.
Nature communications, 9(1), 2252-2252 (2018-06-15)
Angiopoietin-like 4 (ANGPTL4) is an endogenous inhibitor of lipoprotein lipase that modulates lipid levels, coronary atherosclerosis risk, and nutrient partitioning. We hypothesize that loss of ANGPTL4 function might improve glucose homeostasis and decrease risk of type 2 diabetes (T2D). We
ANGPTL4 variants E40K and T266M are associated with lower fasting triglyceride levels and predicts cardiovascular disease risk in Type 2 diabetic Tunisian population.
Abid K
Lipids in Health and Disease (2016)
Angiopoietin-like-4 and minimal change disease
Gabriel C
PLoS ONE (2017)
Angiopoietin-like protein 4 serum levels and gene polymorphisms are associated with large artery atherosclerotic stroke.
He XW
Journal of the Neurological Sciences (2016)
Angiopoietin-Like Protein 4 and Postprandial Skeletal Muscle Lipid Metabolism in Overweight and Obese Prediabetics.
van der Kolk BW
The Journal of Clinical Endocrinology and Metabolism (2016)

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