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RAB0024

Sigma-Aldrich

Human β-2 Microglobulin ELISA Kit

for serum, plasma, cell culture supernatant and urine

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Synonym(s):
B2M

species reactivity

human

packaging

kit of 96 wells (12 strips x 8 wells)

technique(s)

ELISA: suitable
capture ELISA: suitable

input

sample type cell culture supernatant(s)
sample type plasma
sample type serum
sample type urine

assay range

inter-assay cv: <12%
intra-assay cv: <10%
sensitivity: 6 pg/mL
standard curve range: 15.6-1000 pg/mL

detection method

colorimetric

shipped in

wet ice

storage temp.

−20°C

Gene Information

human ... B2M(567)

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RAB0026RAB0600RAB0461
Human BDNF ELISA Kit for serum, plasma, cell culture supernatant and urine

Sigma-Aldrich

RAB0026

Human BDNF ELISA Kit

packaging

kit of 96 wells (12 strips x 8 wells)

packaging

kit of 96 wells (12 strips x 8 wells)

packaging

kit of 96 wells (12 strips x 8 wells)

packaging

kit of 96 wells (12 strips x 8 wells)

technique(s)

ELISA: suitable, capture ELISA: suitable

technique(s)

ELISA: suitable, capture ELISA: suitable

technique(s)

ELISA: suitable

technique(s)

ELISA: suitable, capture ELISA: suitable

input

sample type cell culture supernatant(s)
sample type plasma
sample type serum
sample type urine

input

sample type plasma
sample type serum
sample type cell culture supernatant(s)
sample type urine

input

sample type cell culture supernatant(s)
sample type plasma
sample type serum

input

sample type serum
sample type plasma
sample type cell culture supernatant(s)

assay range

inter-assay cv: <12%
intra-assay cv: <10%
sensitivity: 6 pg/mL
standard curve range: 15.6-1000 pg/mL

assay range

inter-assay cv: <12%
intra-assay cv: <10%
sensitivity: 80 pg/mL
standard curve range: 0.066-16 ng/mL

assay range

inter-assay cv: <12%
intra-assay cv: <10%
sensitivity: 4 ng/mL
standard curve range: 12.29-3000 ng/mL

assay range

inter-assay cv: <12%
intra-assay cv: <10%
sensitivity: 15 pg/mL
standard curve range: 8.23-6000 pg/mL

detection method

colorimetric

detection method

colorimetric

detection method

colorimetric

detection method

colorimetric

General description

β2-microglobulin (B2M) is a non-glycosylated polypeptide composed of 99 amino acids. It is present on the surface of all nucleated cells. The protein is characterized with a single polypeptide chain, which is linked noncovalently to major histocompatibility complex (MHC) class I cell surface antigen. The gene coding for B2M is mapped to human chromosome 15q.
The Human BDNF ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of human BDNF in serum, plasma, cell culture supernatants and urine.

Immunogen

Recombinant Human β-2-Microglobulin

Application

For research use only. Not for use in diagnostic procedures.
Please refer to the attached General ELISA KIT Procedure (sandwich, competitive & Indirect ELISA)

Biochem/physiol Actions

β2-microglobulin (B2M) plays a vital role in cell survival, proliferation and metastasis in various types of cancer. Elevated concentration of β-2-microglobulin (B2M) can be considered as a prognostic marker for hemophagocytic lymphohistiocytosis (HLH). Elevated concentration of circulating B2M, due to renal dysfunction and long term hemodialysis, leads to dialysis-related amyloidosis. Overexpression of serum B2M is observed in hepatitis C virus (HCV) related chronic liver diseases, therefore it might act as a potential marker for the HCV disease progression towards cirrhosis and carcinoma. Missense mutation in the B2M gene results in familial hypercatabolic hypoproteinemia (FHH).

Other Notes

A sample Certificate of Analysis is available for this product.
Please type the word sample in the text box provided for lot number.

Kit Components Also Available Separately

Product No.
Description
SDS

  • RABELADBELISA 5X Assay/Sample Diluent Buffer B (Item E1)SDS

  • RABELADCELISA 1X Assay/Sample Diluent Buffer C (Item L)SDS

  • RABSTOP3ELISA Stop Solution (Item I)SDS

  • RABTMB3ELISA Colorimetric TMB Reagent (HRP Substrate, Item H)SDS

  • RABWASH420X Wash Buffer (Item B)SDS

Pictograms

Corrosion

Signal Word

Warning

Hazard Statements

Precautionary Statements

Hazard Classifications

Met. Corr. 1

Storage Class Code

8A - Combustible, corrosive hazardous materials

WGK

WGK 3


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The Prognostic Significance of Beta2 Microglobulin in Patients with Hemophagocytic Lymphohistiocytosis.
Jiang T
Disease Markers, 1523959 (2016)
Uncovering the Early Assembly Mechanism for Amyloidogenic ?2-Microglobulin Using Cross-linking and Native Mass Spectrometry.
Hall Z
The Journal of Biological Chemistry, 291(9), 4626-4637 (2016)
Kinetics of FcRn-mediated recycling of IgG and albumin in human: pathophysiology and therapeutic implications using a simplified mechanism-based model.
Kim J
Clinical Immunology (Orlando, Fla.), 122(2), 146-155 (2007)
Serum Beta-2 Microglobulin: a Possible Marker for Disease Progression in Egyptian Patients with Chronic HCV Related Liver Diseases.
Ouda SM
Asian Pacific Journal of Cancer Prevention, 16(17), 7825-7829 (2015)
Lengths of chromosomal segments conserved since divergence of man and mouse.
Nadeau JH and Taylor BA.
Proceedings of the National Academy of Sciences of the USA, 81(3), 814-818 (1984)

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