MilliporeSigma
All Photos(2)

RAB0055

Sigma-Aldrich

Mouse MCP-1 / CCL2 ELISA Kit

for serum, plasma and cell culture supernatant

NACRES:
NA.32

species reactivity

mouse

packaging

kit of 96 wells (12 strips x 8 wells)

technique(s)

ELISA: suitable
capture ELISA: suitable

input

sample type plasma
sample type serum
sample type cell culture supernatant(s)

assay range

inter-assay cv: <12%
intra-assay cv: <10%
sensitivity: 3 pg/mL
standard curve range: 2.74-2000 pg/mL

detection method

colorimetric

shipped in

wet ice

storage temp.

−20°C

Gene Information

mouse ... Ccl2(20296)

General description

The Mouse MCP-1 ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of mouse MCP-1 in serum, plasma and cell culture supernatants.

Immunogen

Recombinant Mouse MCP1

Application

For research use only. Not for use in diagnostic procedures.
Please refer to the attached General ELISA KIT Procedure (sandwich, competitive & Indirect ELISA)

Biochem/physiol Actions

Monocyte chemoattractant protein-1 (MCP-1) or chemokine (C-C motif) ligand 2 (CCL2) is expressed in the suprachiasmatic nuclei (SCN) in the hypothalamus of mice. It has a role in the survival and proliferation of microglia. MCP-1 has been shown to activate GTPases like cell division control protein 42 (CDC42) and Ras-related C3 botulinum toxin substrate 1 (Rac1).

Other Notes

A sample Certificate of Analysis is available for this product.
Please type the word sample in the text box provided for lot number.

Kit Components Also Available Separately

Product No.
Description
SDS

  • RABELADAELISA 1X Assay/Sample Diluent Buffer A (Item D1)

  • RABELADBELISA 5X Assay/Sample Diluent Buffer B (Item E1)

  • RABSTOP3ELISA Stop Solution (Item I)

  • RABTMB3ELISA Colorimetric TMB Reagent (HRP Substrate, Item H)

  • RABWASH420X Wash Buffer (Item B)

Pictograms

Corrosion

Signal Word

Warning

Hazard Statements

Precautionary Statements

Hazard Classifications

Met. Corr. 1

Storage Class Code

8A - Combustible, corrosive hazardous materials

WGK

WGK 3

Certificate of Analysis

Enter Lot Number to search for Certificate of Analysis (COA).

Certificate of Origin

Enter Lot Number to search for Certificate of Origin (COO).

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CCL2 mediates the circadian response to low dose endotoxin.
Duhart JM
Neuropharmacology (2016)
Vascular Stem/Progenitor Cell Migration Induced by Smooth Muscle Cell-Derived Chemokine (C-C Motif) Ligand 2 and Chemokine (C-X-C motif) Ligand 1 Contributes to Neointima Formation.
Yu B
Stem Cells (2016)
Guangyao Liu et al.
International immunopharmacology, 66, 354-361 (2018-12-07)
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Michael Bauer et al.
Science advances, 7(51), eabl7150-eabl7150 (2021-12-18)
Antiviral defense and virus exclusion from the cell nucleus restrict foreign nucleic acid influx and infection. How the genomes of DNA viruses evade cytosolic pattern recognition and cross the nuclear envelope is incompletely understood. Here, we show that the virion

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