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RAB0082

Sigma-Aldrich

Human CD163 ELISA Kit

for serum, plasma, cell culture supernatant and urine

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NACRES:
NA.32

species reactivity

human

packaging

kit of 96 wells (12 strips x 8 wells)

technique(s)

ELISA: suitable
capture ELISA: suitable

input

sample type plasma
sample type cell culture supernatant(s)
sample type urine
sample type serum

assay range

inter-assay cv: <12%
intra-assay cv: <10%
sensitivity: 30 pg/mL
standard curve range: 32.77-8000 pg/mL

detection method

colorimetric

shipped in

wet ice

storage temp.

−20°C

Gene Information

human ... CD163(9332)

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Human CD163 ELISA Kit

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Gene Information

human ... CD163(9332)

Gene Information

human ... CD14(929)

Gene Information

human ... DPP4(1803)

Gene Information

human ... CDH3(1001)

species reactivity

human

species reactivity

human

species reactivity

human

species reactivity

human

assay range

inter-assay cv: <12%
intra-assay cv: <10%
sensitivity: 30 pg/mL
standard curve range: 32.77-8000 pg/mL

assay range

inter-assay cv: <12%
intra-assay cv: <10%
sensitivity: 6 pg/mL
standard curve range: 8.23-6000 pg/mL

assay range

inter-assay cv: <12%
intra-assay cv: <10%
sensitivity: 25 pg/mL
standard curve range: 32.77-8000 pg/mL

assay range

inter-assay cv: <12%
intra-assay cv: <10%
sensitivity: 20 pg/mL
standard curve range: 24.7-18000 pg/mL

shipped in

wet ice

shipped in

wet ice

shipped in

wet ice

shipped in

wet ice

detection method

colorimetric

detection method

colorimetric

detection method

colorimetric

detection method

colorimetric

General description

The Human CD163 (also known as hemoglobin scavenger receptor) ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of human CD163 in serum, plasma, cell culture supernatants and urine.

Immunogen

Recombinant Human CD163

Application

For research use only. Not for use in diagnostic procedures.
Please refer to the attached General ELISA KIT Procedure (sandwich, competitive & Indirect ELISA)
Human CD163 ELISA kit has been used to measure the levels of CD163 in plasma samples and cell culture supernatants.

Biochem/physiol Actions

Cluster of differentiation 163 (CD163) is a scavenger receptor. It is expressed on the surface of activated macrophages. This protein modulates anti-inflammatory functions and has a role in hemoglobin-haptoglobin clearance. Various cytokines influence the expression of CD163. It may be associated with hepatocellular carcinoma.

Other Notes

A sample Certificate of Analysis is available for this product.
Please type the word sample in the text box provided for lot number.

Kit Components Also Available Separately

Product No.
Description
SDS

  • RABELADAELISA 1X Assay/Sample Diluent Buffer A (Item D1)SDS

  • RABELADBELISA 5X Assay/Sample Diluent Buffer B (Item E1)SDS

  • RABSTOP3ELISA Stop Solution (Item I)SDS

  • RABTMB3ELISA Colorimetric TMB Reagent (HRP Substrate, Item H)SDS

  • RABWASH420X Wash Buffer (Item B)SDS

pictograms

Corrosion

signalword

Warning

hcodes

Hazard Classifications

Met. Corr. 1

Storage Class

8A - Combustible, corrosive hazardous materials

wgk_germany

WGK 3


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Monocyte-lymphocyte cross-communication via soluble CD163 directly links innate immune system activation and adaptive immune system suppression following ischemic stroke
Grant C
Scientific Reports (2017)
Martin Hoenigl et al.
Clinical infectious diseases : an official publication of the Infectious Diseases Society of America, 69(4), 676-686 (2018-11-13)
Despite effective antiretroviral therapy (ART), human immunodeficiency virus (HIV) infection remains associated with higher morbidity and mortality, driven, in part, by increased inflammation. Our objective was to identify associations between levels of plasma biomarkers of chronic inflammation, microbial translocation, and
Clinical significance of soluble CD163 in polymyositis-related or dermatomyositis-related interstitial lung disease.
Enomoto Y
Arthritis Research & Therapy (2017)
A Ross Eckard et al.
HIV medicine, 15(9), 537-546 (2014-03-22)
HIV-infected patients on antiretroviral therapy (ART) have an increased cardiovascular disease (CVD) risk as a result of heightened inflammation and immune activation, despite at times having normal lipids and few traditional risk factors. Biomarkers are needed to identify such patients
Grant C O'Connell et al.
Scientific reports, 7(1), 12940-12940 (2017-10-13)
CD163 is a scavenger receptor expressed on innate immune cell populations which can be shed from the plasma membrane via the metalloprotease ADAM17 to generate a soluble peptide with lympho-inhibitory properties. The purpose of this study was to investigate CD163

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