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MilliporeSigma

RAB0093

Human CNTF ELISA Kit

for serum, plasma, cell culture supernatant and urine

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1 KIT

$608.00

$608.00


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About This Item

NACRES:
NA.32
UNSPSC Code:
41116158

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Product Name

Human CNTF ELISA Kit, for serum, plasma, cell culture supernatant and urine

species reactivity

human

packaging

kit of 96 wells (12 strips x 8 wells)

technique(s)

ELISA: suitable
capture ELISA: suitable

input

sample type serum
sample type plasma
sample type urine
sample type cell culture supernatant(s)

assay range

inter-assay cv: <12%
intra-assay cv: <10%
sensitivity: 200 pg/mL
standard curve range: 0.823-200 ng/mL

detection method

colorimetric

shipped in

wet ice

storage temp.

−20°C

Gene Information

human ... CNTF(1270)

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RAB0094RAB0330RAB0196
species reactivity

human

species reactivity

rat

species reactivity

human

species reactivity

human

Gene Information

human ... CNTF(1270)

Gene Information

rat ... Cntf(12803)

Gene Information

human ... KITLG(4254)

Gene Information

human ... FSTL3(10272)

input

sample type serum
sample type plasma
sample type urine
sample type cell culture supernatant(s)

input

sample type cell culture supernatant(s)
sample type serum
sample type plasma

input

sample type serum
sample type urine
sample type cell culture supernatant(s)
sample type plasma

input

sample type plasma
sample type urine
sample type cell culture supernatant(s)
sample type serum

assay range

inter-assay cv: <12%
intra-assay cv: <10%
sensitivity: 200 pg/mL
standard curve range: 0.823-200 ng/mL

assay range

inter-assay cv: <12%
intra-assay cv: <10%
sensitivity: 10 pg/mL
standard curve range: 8.23-2000 pg/mL

assay range

inter-assay cv: <12%
intra-assay cv: <10%
sensitivity: 2 pg/mL
standard curve range: 2.74-2000 pg/mL

assay range

inter-assay cv: <12%
intra-assay cv: <10%
sensitivity: 250 pg/mL
standard curve range: 204.8-50000 pg/mL

detection method

colorimetric

detection method

colorimetric

detection method

colorimetric

detection method

colorimetric

technique(s)

ELISA: suitable, capture ELISA: suitable

technique(s)

ELISA: suitable, capture ELISA: suitable

technique(s)

ELISA: suitable, capture ELISA: suitable

technique(s)

ELISA: suitable, capture ELISA: suitable

Application

For research use only. Not for use in diagnostic procedures.
Please refer to the attached General ELISA KIT Procedure (sandwich, competitive & Indirect ELISA)

Biochem/physiol Actions

Ciliary neurotrophic factor (CNTF) is part of the cytokine family. It signals through the glycoprotein 130 and LIF receptor β subunit. The protein has neuroprotective effects and activates astrocytes. CNTF also has a role in the differentiation of neural stem cells and is involved in neurotransmitter synthesis.[1][2][3][4]

General description

The Human CNTF ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of human CNTF in serum, plasma, cell culture supernatants and urine.

Immunogen

Recombinant Human CNTF

Other Notes

A sample Certificate of Analysis is available for this product.
Please type the word sample in the text box provided for lot number.

pictograms

Corrosion

signalword

Warning

hcodes

Hazard Classifications

Met. Corr. 1

Storage Class

8A - Combustible corrosive hazardous materials


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The neuroprotective agent CNTF decreases neuronal metabolites in the rat striatum: an in vivo multimodal magnetic resonance imaging study
Maria-Angeles
Journal of Cerebral Blood Flow and Metabolism (2015)
Role of ciliary neurotrophic factor in the proliferation and differentiation of neural stem cells.
Ding J
Journal of Alzheimer'S Disease (2013)
Ciliary neurotrophic factor (CNTF): New facets of an old molecule for treating neurodegenerative and metabolic syndrome pathologies.
Pasquin S
Cytokine & Growth Factor Reviews (2015)
Neuroprotective properties of ciliary neurotrophic factor on retinoic acid (RA)-predifferentiated SH-SY5Y neuroblastoma cells.
Wang K
Folia Neuropathologica (2014)
I-Mo Fang et al.
Journal of ocular pharmacology and therapeutics : the official journal of the Association for Ocular Pharmacology and Therapeutics, 30(9), 757-770 (2014-08-15)
To investigate the efficacy and mechanisms of non-c-Myc induced pluripotent stem cell (iPSC) transplantation in a rat model of retinal oxidative damage. Paraquat was intravitreously injected into Sprague-Dawley rats. After non-c-Myc iPSC transplantation, retinal function was evaluated by electroretinograms (ERGs).

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